Micropatterned Hydrogel Surface with High-Aspect-Ratio Features for Cell Guidance and Tissue Growth
Micropatterned Hydrogel Surface with High-Aspect-Ratio Features for Cell Guidance and Tissue Growth
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DOI：
10.1021/acsami.5b12268
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发表时间：
2016-08-31
影响因子：
9.5
通讯作者：
Aizenberg, Joanna
中科院分区：
文献类型：
--
作者：
Hu, Yuhang;You, Jin-Oh;Aizenberg, Joanna
表面形貌已被引入作为协调细胞选择、生长、形态和分化的新工具。迄今为止，用于制造这种结构表面的材料大多是刚性和不可渗透的。而水凝胶由于其生物相容性、柔软性和高渗透性，被证明是一种更好的细胞培养合成介质。在此，我们制作了一个聚（2-羟乙基甲基丙烯酸酯）（pHEMA）水凝胶基板与高纵横比的表面微观特征。这种结构表面可以有效地引导人骨髓间充质干细胞（HMSCs）的方向和形状。值得注意的是，在平坦的水凝胶表面上，细胞变圆，而在微孔板图案化的水凝胶表面上，细胞伸长并沿沿着平行于板的方向排列。微孔板厚2 μ m，高20 μ m，宽10-50 μ m。板间距5-15 μ m，柱间距5 μ m。细胞体的伸长在板间距窄、板宽的模式上更为明显。细胞表现为软固体。表面能和弹性能之间的竞争决定了结构化表面上细胞的形状。具有表面结构的柔软可渗透水凝胶支架也被证明对于长期细胞培养是可行的，并且可以用于产生具有精细调整的细胞形态和跨几厘米尺寸的对齐的互连组织。
Surface topography has been introduced as a new tool to coordinate cell selection, growth, morphology, and differentiation. The materials explored so far for making such structural surfaces are mostly rigid and impermeable. Hydrogel, on the other hand, was proved a better synthetic media for cell culture because of its biocompatibility, softness, and high permeability. Herein, we fabricated a poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogel substrate with high-aspect-ratio surface microfeatures. Such structural surface could effectively guide the orientation and shape of human mesenchymal stem cells (HMSCs). Notably, on the flat hydrogel surface, cells rounded up, whereas on the microplate patterned hydrogel surface, cells elongated and aligned along the direction parallel to the plates. The microplates were 2 mu m thick, 20 mu m tall, and 10-50 mu m wide. The interplate spacing was 5-15 mu m, and the intercolumn spacing was 5 mu m. The elongation of cell :body was more pronounced on the patterns with narrower interplate spacing and wider plates. The cells behaved like soft solid. The competition between surface energy and elastic energy defined the shape of the cells on the structured surfaces. The soft permeable hydrogel scaffold with surface structures was also demonstrated as being viable for long-term cell culture, and could be used to generate interconnected tissues with finely tuned cell morphology and alignment across a few centimeter sizes.