Ginsenoside Rb1 protects porcine oocytes against methylglyoxal damage thus it improves the quality of parthenogenetic activation and in vitro fertilization embryos
Ginsenoside Rb1 protects porcine oocytes against methylglyoxal damage thus it improves the quality of parthenogenetic activation and in vitro fertilization embryos
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人参皂苷 Rb1 保护猪卵母细胞免受甲基乙二醛损伤，从而提高孤雌激活和体外受精胚胎的质量
DOI：
10.1002/tox.23063
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发表时间：
2020-11-25
影响因子：
4.5
通讯作者：
Kang, Jin-Dan
中科院分区：
文献类型：
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作者：
Luo, Zhao-Bo;Xuan, Mei-Fu;Kang, Jin-Dan
人参是一种功能性食品，作为营养品和药用辅料已被广泛应用。人参皂苷Rb1是人参的主要活性成分，具有特异的抗细胞凋亡和抗氧化活性。丙酮醛（MGO）是葡萄糖代谢的中间产物之一，在高糖食物或碳酸饮料中极易被人体吸收。它可能参与多种体内有害过程。然而，它还没有充分探讨的MGO诱导的卵母细胞损伤的影响。本研究发现，MGO诱导的DNA损伤和线粒体功能障碍导致猪卵母细胞成熟失败，孤雌激活（PA）和体外受精（IVF）胚胎的体外发育能力低下。相反，补充Rb1恢复了成熟率，并提高了PA和IVF胚胎的体外发育能力。Rb1还能降低猪卵母细胞活性氧的产生，提高ATP含量和线粒体膜电位，并刺激囊胚中多能性基因的表达。本研究结果揭示了人参皂苷Rb1对甲基乙二醛的细胞毒性具有保护作用，为人参功能性食品对生殖系统的保护提供了新的视角。
Panax ginseng, a functional food, has been widely used as an edible nourishment and medicinal supplement. Ginsenoside Rb1 is a major bioactive ingredient of ginseng, which shows very specific anti-apoptosis and anti-oxidant activities. Methylglyoxal (MGO) is one of intermediate products of glucose metabolism, which is absorbed easily from high sugar foods or carbonated beverages. It may involve in a variety of detrimental processes in vivo. However, it has not been fully explored the effects of ginsenoside Rb1 on MGO-induced oocytes damage. This study found that MGO-induced DNA damage and mitochondrial dysfunction result in the failure of porcine oocytes maturation and low in vitro development capacity of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos. Conversely, Rb1 supplementation recovered the rate of maturation, and improved in vitro development capacity of PA and IVF embryos. Rb1 also provided porcine oocytes a lower level of reactive oxygen species production, higher level of ATP content and mitochondrial membrane potential, and stimulated pluripotency gene expression in blastocysts. The findings of this study reveal ginsenoside Rb1 protects porcine oocyte from the cytotoxicity effects of methylglyoxal and provides novel perspectives for the protection of reproduction system by functional food of ginseng.