Sequential activation of Notch family receptors during mouse spermatogenesis
Sequential activation of Notch family receptors during mouse spermatogenesis
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DOI：
10.1046/j.1440-169x.2003.00670.x
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发表时间：
2003-02-01
影响因子：
2.5
通讯作者：
Marunouchi, T
中科院分区：
文献类型：
--
作者：
Mori, S;Kadokawa, Y;Marunouchi, T
采用免疫组织化学方法检测Notch家族受体在小鼠精子发生过程中的表达。整个精原细胞，精母细胞和精子细胞的细胞质显示对Notch 1，2和4的细胞外结构域的抗体染色。相反，精原细胞的细胞核显示用针对Notch 3的细胞内结构域的抗体染色，精母细胞和精子细胞的细胞核显示用针对Notch 1和4的细胞内结构域的抗体染色。在白消安处理的小鼠精原细胞再生过程中，所有增殖细胞的细胞核均显示Notch3胞内结构域染色。Western blot分析表明，Notch1和Notch3的胞内结构域的分子量分布在细胞核中，小于胞浆中的分子量。这与Notch的胞内结构域在细胞质中被切割并易位到细胞核的理论一致。这些结果表明，不同的Notch信号在小鼠精子发生过程中顺序激活，并控制生精干细胞的增殖和分化。
The expression pattern of Notch family receptors during mouse spermatogenesis was examined by immunohistochemistry. The entire cytoplasm of spermatogonia, spermatocytes and spermatids showed staining with antibodies against extracellular domains of Notch1, 2 and 4. In contrast, the nuclei of spermatogonia showed staining with an antibody against the intracellular domain of Notch3, and the nuclei of spermatocytes and spermatids showed staining with antibodies against the intracellular domains of Notch1 and 4. During regeneration of spermatogonia in busulfan-treated mice, the nuclei of all proliferating cells showed staining for the intracellular domain of Notch3. Western blot analysis showed that the molecular weights of the intracellular domains of Notch1 and 3 localizing in the nuclear fraction were smaller than those in the cytoplasmic fraction. This was consistent with the theory that the intracellular domain of Notch was cleaved in the cytoplasm and translocated to the nucleus. These results suggest that different Notch signals are sequentially activated during mouse spermatogenesis and control the proliferation and differentiation of spermatogenic stem cells.