Drilling into the Metabolomics to Enhance Insight on Corn and Wheat Responses to Molybdenum Trioxide Nanoparticles
Drilling into the Metabolomics to Enhance Insight on Corn and Wheat Responses to Molybdenum Trioxide Nanoparticles
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DOI：
10.1021/acs.est.1c00803
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发表时间：
2021-05-27
影响因子：
11.4
通讯作者：
Keller, Arturo A.
中科院分区：
文献类型：
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作者：
Huang, Xiangning;Cervantes-Aviles, Pabel;Keller, Arturo A.
代谢组学是一个新兴的工具，了解纳米技术的潜在影响，特别是对农业。虽然钼（Mo）是一种已知的植物微量营养素，但对其代谢扰动知之甚少。在这里，玉米和小麦幼苗暴露于MoO3纳米粒子（NPs）和相应的生物可利用的Mo6+离子在中等和过量的水平，通过根暴露。在生理上，玉米对钼更敏感，积累量是小麦的3.63倍。相比之下，代谢组学研究表明，玉米叶片中有21种代谢物失调，小麦叶片中有53种。与玉米叶片相比，小麦叶片中多了五条代谢途径。除了总体代谢组学分析外，我们还分析了单个代谢物类别（例如，氨基酸、有机酸等），在植物组织中产生额外的失调代谢物：玉米为7，小麦为7。其中大部分是氨基酸以及一些糖。额外的显著失调的代谢物（例如，天冬酰胺，果糖，还原型谷胱甘肽，甘露糖），由于钼NP暴露，在玉米和小麦中，通过采用个别代谢物组分析。因此，对各组进行靶向代谢物分析对于发现其他重要代谢物非常重要。我们证明了代谢组学的价值，研究早期阶段的植物对NP暴露的反应。
Metabolomics is an emerging tool to understand the potential implications of nanotechnology, particularly for agriculture. Although molybdenum (Mo) is a known plant micronutrient, little is known of its metabolic perturbations. Here, corn and wheat seedlings were exposed to MoO3 nanoparticles (NPs) and the corresponding bioavailable Mo6+ ion at moderate and excessive levels through root exposures. Physiologically, corn was more sensitive to Mo, which accumulated up to 3.63 times more Mo than wheat. In contrast, metabolomics indicated 21 dysregulated metabolites in corn leaves and 53 in wheat leaves. Five more metabolomic pathways were perturbed in wheat leaves compared to corn leaves. In addition to the overall metabolomics analysis, we also analyzed individual metabolite classes (e.g., amino acids, organic acids, etc.), yielding additional dysregulated metabolites in plant tissues: 7 for corn and 7 for wheat. Most of these were amino acids as well as some sugars. Additional significantly dysregulated metabolites (e.g., asparagine, fructose, reduced glutathione, mannose) were identified in both corn and wheat, due to Mo NP exposure, by employing individual metabolite group analysis. Targeted metabolite analysis of individual groups is thus important for finding additional significant metabolites. We demonstrate the value of metabolomics to study early stage plant responses to NP exposure.