Dopamine and [D-ALA2, D-Leu5]enkephalin inhibit the electrically stimulated neurohypophyseal release of vasopressin in vitro: evidence for calcium-dependent opiate action
Dopamine and [D-ALA2, D-Leu5]enkephalin inhibit the electrically stimulated neurohypophyseal release of vasopressin in vitro: evidence for calcium-dependent opiate action
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多巴胺和 [D-ALA2、D-Leu5] 脑啡肽在体外抑制电刺激神经垂体释放加压素：钙依赖性阿片类药物作用的证据
DOI：
10.1523/jneurosci.02-01-00078.1982
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
M. Forsling
中科院分区：
文献类型：
--
作者：
S. Lightman;L. Iversen;M. Forsling
多巴胺和脑啡肽激动剂对电刺激离体神经叶抗利尿激素分泌的影响进行了评估。多巴胺（10(-6)M）和多巴胺激动剂ADTN（2-氨基- 6,7-二羟基-1,2,3,4-四氢萘；10(-6)M）均可显著降低加压素的刺激释放。多巴胺拮抗剂舒必利（10(-6)M）阻止了抗利尿激素分泌的减少，[D-Ala2， D-Leu5]脑啡肽（DADLE）也抑制抗利尿激素的释放，其作用与ADTN的作用是互补的。阿片拮抗剂纳洛酮可阻止DADLE的作用，并且对培养基中的钙含量敏感。低钙浓度显著增强了DADLE的抑制作用，而高浓度则消除了其抑制作用。这些发现为多巴胺和脑啡肽在神经叶水平上的直接抑制作用提供了证据，并表明脑啡肽的作用可能与钙敏感的神经分泌事件密切相关。
The effects of both dopamine and enkephalin agonists were assessed on electrically stimulated vasopressin secretion from the isolated neural lobe. Both dopamine (10(-6) M) and the dopamine agonist ADTN (2-amino- 6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene; 10(-6) M) significantly reduced the stimulated release of vasopressin. This reduction in vasopressin secretion was prevented by the dopamine antagonist sulpiride (10(-6) M). [D-Ala2, D-Leu5]Enkephalin (DADLE) also inhibited vasopressin release and its effect was additive to that of ADTN. The effect of DADLE was prevented by the opiate antagonist naloxone and was also sensitive to the calcium content of the medium. Low calcium concentrations markedly increased the inhibitory effect of DADLE, while high concentrations abolished its effect. These findings provide evidence of a direct inhibitory action of both dopamine and enkephalins at the level of the neural lobe and suggest that the effect of enkephalins may be intimately related to calcium-sensitive neurosecretory events.