REPETITIVE PROCUREMENT OF MATURE GAMETES FROM INDIVIDUAL SEA STARS AND SEA-URCHINS
REPETITIVE PROCUREMENT OF MATURE GAMETES FROM INDIVIDUAL SEA STARS AND SEA-URCHINS
复制标题
DOI：
10.1083/jcb.57.3.879
复制
发表时间：
1973-01-01
影响因子：
7.8
通讯作者：
FUSELER, JW
中科院分区：
文献类型：
--
作者：
FUSELER, JW
从海星（1，2）和海胆（3，4，6）中获得成熟配子的各种常规方法非常适合于需要大量或混合大量来自不同个体的配子的实验。然而，通常希望在良好控制的条件下对少数细胞进行实验，并且来自单个个体。为此，我设计了一种方法，可以从一只动物身上重复获得健康的棘皮动物卵，持续时间为一周或更长。此外，对于观察和测量的各种显微镜方法，人们可能希望选择和使用其卵具有很少光散射或双折射内含物的雌性。鸡蛋的光学清晰度对于一个人来说是相当均匀的，但从一个人到另一个人可能会有很大的差异。
The various conventional methods of procuring ripe gametes from sea stars (1, 2), and sea urchins (3, 4, 6) are well suited for experiments in which large quantities or pooled lots of gametes from different individuals are required. It is often desirable, however, to perform experiments on a few cells, under well controlled conditions and from a single individual. For this purpose I have devised methods which permit repetitive procurement of healthy echinoderm eggs from a single animal for periods of a week or more. In addition, for various microscopic methods of observation and measurement, one may wish to select and use females whose eggs possess few light scattering or birefringent inclusions. The degree of optical clarity of eggs is fairly uniform for one individual, but can vary widely from one individual to another.