Nasal lymphoid tissue (NALT) as a mucosal immune inductive site
Nasal lymphoid tissue (NALT) as a mucosal immune inductive site
复制标题
DOI：
10.1046/j.1365-3083.1997.d01-159.x
复制
发表时间：
1997-11-01
影响因子：
3.7
通讯作者：
Russell, MW
中科院分区：
文献类型：
--
作者：
Wu, HY;Nguyen, HH;Russell, MW
鼻内免疫是诱导粘膜免疫的一种非常有效的途径，但负责调节和传播这些反应的细胞机制尚不完全清楚。作者利用机械分离获得的高纯度鼻淋巴组织（NALT）细胞，研究了NALT作为粘膜诱导部位的作用。与唾液腺（SG）和小肠固有层（LP）淋巴细胞相比，NALT细胞如Peyer's patch （PP）细胞体积更小，颗粒状更少。从免疫小鼠中分离的NALT细胞含有抗原特异性抗体分泌细胞（ASC），主要是免疫球蛋白（Ig）A同型，类似于在时间过程研究中从唾液腺中回收的细胞。然而，在ELISPOT检测中，由NALT细胞形成的小尺寸斑点比例较高，表明这些细胞是唾液腺中发现的分化程度较低的前体。免疫后，IgA ASC出现在NALT以及粘膜效应部位SG和LP中，但在另一个粘膜诱导部位PP中没有或很少出现。相反，胃内免疫后，IgA ASC在PP、SG和LP中检测到，而在NALT中没有或很少检测到。此外，免疫后，来自NALT而非PP的淋巴细胞对培养中的特定抗原有增殖反应。这些发现表明NALT在粘膜效应位点作为IgA抗体反应的诱导位点。
Intranasal (i.n.) immunization is a very effective route for inducing mucosal immunity, but the cellular mechanism responsible for regulating and disseminating these responses is not fully understood. The authors studied the role of nasal lymphoid tissue (NALT) as a mucosal inductive site by using highly purified NALT cells obtained by a new method of mechanical isolation. The NALT cells, like Peyer's patch (PP) cells, were smaller in size and less granular than lymphocytes from salivary glands (SG) and small intestinal lamina propria (LP). The NALT cells isolated from i.n. immunized mice contained antigen-specific antibody-secreting cells (ASC) predominantly of immunoglobulin (Ig)A isotype, similar to those also recovered from salivary glands in a time course study. However, the higher proportion of smaller sized spots formed by NALT cells in ELISPOT assays suggested that these cells were less differentiated precursors of these found in salivary glands. This was supported by the fact that after i.n. immunization, IgA ASC appeared in NALT, as well as in mucosal effector sites SG and LP, but none or very few in another mucosal inductive site, PP. In contrast, after intragastric (i.g.) immunization, IgA ASC were detected in PP, along with the SG and LP, but none or very few in NALT. Furthermore, after i.n. immunization, lymphocytes from NALT but not PP proliferated in response to the specific antigen in culture. These findings imply that NALT served as an inductive site for IgA antibody responses at mucosal effector sites.