Reductive Dehalogenation of Oligocyclic Phenolic Bromoaromatics by Dehalococcoides mccartyi Strain CBDB1.
Reductive Dehalogenation of Oligocyclic Phenolic Bromoaromatics by Dehalococcoides mccartyi Strain CBDB1.
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DOI：
10.1021/acs.est.5b01401
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发表时间：
2015-07
影响因子：
11.4
通讯作者：
Chao Yang;Anja Kublik;Cindy Weidauer;B. Seiwert;L. Adrian
中科院分区：
文献类型：
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作者：
Chao Yang;Anja Kublik;Cindy Weidauer;B. Seiwert;L. Adrian
麦卡提脱卤球菌能转化许多卤代化合物，并用于生物修复。这种厌氧转化用氯化和简单结构的化合物，如氯代苯、乙烯和乙烷进行了深入的研究。然而，许多卤代低环芳香族化合物在自然界中要么作为天然原料，要么作为药品、杀虫剂或阻燃剂等商业产品的一部分。在这里，我们证明了麦卡氏菌CBDB1菌株还原脱溴两种低环芳香族酚类化合物，四溴双酚A(TBBPA)和溴苯酚蓝(BPB)。菌株CBDB1通过逐步去除所有的溴代取代基，将TBBPA完全转化为双酚A，BPB完全转化为酚红。在用TBBPA培养的培养物中检测到脱溴(但没有细胞生长)。相反，菌株CBDB1在与BPB相互作用时生长，表明该底物被用作有机溴呼吸的电子受体。在培养早期，高剂量的BPB延迟了脱溴并抑制了生长。与BPB相比，TBBPA具有更高的毒性，这可能是由于其较高的亲脂性所致。全细胞提取液的质谱分析表明，还原脱卤酶同源基因CbdBA1092和CbdBA1503编码的两个蛋白质被所用的寡环化合物特异性诱导，而其他蛋白质(如CbdBA84(CbrA))表达下调。
Dehalococcoides mccartyi strains transform many halogenated compounds and are used for bioremediation. Such anaerobic transformations were intensively studied with chlorinated and simply structured compounds such as chlorinated benzenes, ethenes, and ethanes. However, many halogenated oligocyclic aromatic compounds occur in nature as either naturally produced materials or as part of commercial products such as pharmaceuticals, pesticides, or flame retardants. Here, we demonstrate that the D. mccartyi strain CBDB1 reductively debrominated two oligocyclic aromatic phenolic compounds, tetrabromobisphenol A (TBBPA) and bromophenol blue (BPB). The strain CBDB1 completely converted TBBPA to bisphenol A and BPB to phenol red with a stepwise removal of all bromide substituents. Debromination (but no cell growth) was detected in the cultures cultivated with TBBPA. In contrast, strain CBDB1 grew when interacting with BPB, demonstrating that this substrate was used as an electron acceptor for organobromine respiration. High doses of BPB delayed debromination and inhibited growth in the early cultivation phase. A higher toxicity of TBBPA compared with that of BPB might be due to the higher lipophilicity of TBBPA. Mass spectrometric analyses of whole-cell extracts demonstrated that two proteins encoded by the reductive dehalogenase homologous genes CbdbA1092 and CbdbA1503 were specifically induced by the used oligocyclic compounds, whereas others (e.g., CbdbA84 (CbrA)) were downregulated.