Cultivation of Borrelia burgdorferi in dialysis membrane chambers in rat peritonea.
Cultivation of Borrelia burgdorferi in dialysis membrane chambers in rat peritonea.
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DOI：
10.1002/9780471729259.mc12c03s00
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发表时间：
2005-07-01
影响因子：
--
通讯作者：
Caimano, Melissa J
中科院分区：
文献类型：
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作者：
Caimano, Melissa J
为了在其地方性动物流行周期中持续存在，伯氏疏螺旋体必须适应两种截然不同的环境：节肢动物媒介和哺乳动物宿主。因此，快速适应环境变化的能力被认为是螺旋体生存和致病程序的核心。事实上，现在已经确定，蜱的摄食会引发基因表达和蛋白质组成的广泛变化，统称为“宿主适应”，这一过程被认为在整个感染过程中持续存在。然而，疏螺旋体感染的少杆菌性质阻碍了我们在体内研究这种细菌的能力。为了规避这一限制，开发了一种动物模型以获得足够数量的生物体，以直接检查哺乳动物宿主环境中伯氏疏螺旋体的差异基因表达和抗原组成。 DMC 模型可以直接比较宿主适应的伯氏疏螺旋体及其体外培养的对应物。
In order to be sustained within its enzootic cycle, B. burgdorferi must adapt to two strikingly different environments, the arthropod vector and the mammalian host. The ability to rapidly adapt to environmental changes is therefore presumed to be central to spirochete survival and pathogenic programs. Indeed, it has now been well established that tick feeding initiates extensive changes in both gene expression and protein composition, collectively referred to as "host adaptation," a process that is thought to continue throughout infection. The paucibacillary nature of borrelial infections, however, has hampered our ability to study this bacterium in vivo. To circumvent this limitation, an animal model was developed for obtaining sufficient numbers of organisms to directly examine differential gene expression and antigenic composition of B. burgdorferi within the context of the mammalian host. The DMC model allows for a direct comparison of host-adapted B. burgdorferi and their in vitro-cultivated counterparts.