KINETICS OF BONE-RESORBING ACTIVITY IN DEVELOPING PERIAPICAL LESIONS
KINETICS OF BONE-RESORBING ACTIVITY IN DEVELOPING PERIAPICAL LESIONS
复制标题
DOI：
10.1177/00220345910700100901
复制
发表时间：
1991-10-01
影响因子：
7.6
通讯作者：
STASHENKO, P
中科院分区：
文献类型：
--
作者：
WANG, CY;STASHENKO, P
用大鼠模型系统研究根尖周病变中骨吸收活性的存在和动力学。发现病变在第0天和第15天的诱导（“活动期”）之间最迅速地扩大，此后以较慢的速率发生扩大（第20天和第30天，“慢性期”），如通过测量放大的射线照片和自动图像分析所评估的。在第15天获得的根尖周组织的合并提取物含有显著水平的骨吸收活性，如通过预先标记的胎鼠长骨的Ca-45释放所确定的。正常大鼠牙髓和牙周膜无活性。在两个动力学实验中，在第10天（10.4%和11.6%特异性Ca-45释放/mg/mL）和第15天（8.9%）的组织中检测到最高水平的骨吸收活性。此后，第20天（4.4%和6.1%）活动下降至第30天（1.4%和3.3%）接近基线水平。在存在或不存在多粘菌素-B（细菌脂多糖的抑制剂）的情况下，发现相同水平的再吸收活性。这些研究结果表明，骨吸收活动是暂时相关的根尖周骨破坏，并建议该模型可能是有用的介质，参与炎症性骨吸收的发病机制的研究。
The presence and kinetics of bone-resorbing activity in periapical lesions were studied with a rat model system. Lesions were found to expand most rapidly between induction on day 0 and day 15 ("active phase"), with enlargement occurring at a slower rate thereafter (days 20 and 30, "chronic phase"), as assessed by measurement of magnified radiographs and automated image analysis. Pooled extracts of periapical tissues obtained on day 15 contained significant levels of bone-resorbing activity, as determined by Ca-45 release from pre-labeled fetal rat long bones. Normal rat dental pulp and periodontal ligament contained no activity. In two kinetic experiments, highest levels of bone-resorbing activity were detected in tissues on days 10 (10.4% and 11.6% specific Ca-45 release/mg/mL) and 15 (8.9%). Activity declined thereafter on day 20 (4.4% and 6.1%) to near baseline levels by day 30 (1.4% and 3.3%). Identical levels of resorbing activity were found in the presence or absence of polymyxin-B, an inhibitor of bacterial lipopolysaccharide. These findings demonstrate that bone-resorbing activity is temporally related to periapical bone destruction and suggest that this model may be useful for studies of mediators that are involved in the pathogenesis of inflammatory bone resorption.