Expression and colocalization of NMDA receptor and FosB/ΔFosB in sensitive brain regions in rats after chronic morphine exposure
Expression and colocalization of NMDA receptor and FosB/ΔFosB in sensitive brain regions in rats after chronic morphine exposure
复制标题
慢性吗啡暴露后大鼠敏感脑区 NMDA 受体和 FosB/ΔFosB 的表达和共定位
DOI：
10.1016/j.neulet.2015.11.052
复制
发表时间：
2016-02-12
影响因子：
2.5
通讯作者：
Niu, Haichen
中科院分区：
文献类型：
--
作者：
Zhang, Qiang;Liu, Qi;Niu, Haichen
近十年的研究表明，NMDA受体（NMDAR）在阿片诱导的神经和行为可塑性中起重要作用。此外，FosB样蛋白（FosB/Delta FosB）水平的增加被发现与吗啡戒断行为有关。然而，吗啡戒断过程中敏感脑区NMDAR与FosB/Delta FosB之间的关系在很大程度上是未知的。本研究旨在探讨吗啡戒断过程中多个脑区NMDAR的动态变化和FosB/Delta FosB水平的变化，以及它们在敏感脑区是否相关。采用免疫组织化学方法检测吗啡戒断大鼠NMDAR和FosB/Delta fosB的表达。NMDAR和FosB/Delta FosB在杏仁核核心（AcbC）、杏仁核壳（AcbSh）、中央杏仁核被囊部（CeC）、腹侧被盖区（VTA）和扣带皮层（Cg）的表达明显增加。免疫荧光双标记表明NMDAR与Fos/Delta FosB共定位于这五个区域。这些结果表明，吗啡戒断过程中NMDAR和Fos/Delta FosB介导的多个表型区域，如动机（AcbC，AcbSh）、边缘（CeC，VTA）和执行（Cg）系统通路，可能是NMDAR和Fos/Delta FosB影响吗啡戒断行为的主要靶点。(C)由Elsevier爱尔兰Ltd.出版
Research in the last decade demonstrated that the NMDA receptor (NMDAR) has an important role in opiate-induced neural and behavioral plasticity. In addition, increased levels of FosB-like proteins (FosB/Delta FosB) were found to be related to morphine withdrawal behaviors. However, the relationship between NMDAR and FosB/Delta FosB in sensitive brain regions during morphine withdrawal is largely unknown. In this study, we aimed to investigate NMDAR dynamics and FosB/Delta FosB levels in multiple brain regions and whether they are related in sensitive brain regions during morphine abstinence. Quantitative immunohistochemistry was adopted to test NMDAR and FosB/Delta fosB levels during morphine withdrawal in rats. Increased NMDAR and FosB/Delta FosB levels were found in the nucleus accumbens core (AcbC), nucleus accumbens shell (AcbSh), central amygdaloid nucleuscapsular part (CeC), ventral tegmental area (VTA) and cingulate cortex (Cg). Double-immunofluorescence labeling indicated that NMDAR colocalized with Fos/Delta FosB in these five regions. These results suggest that multiple phenotypic regions are mediated by NMDAR and Fos/Delta FosB during morphine withdrawal, such as the motivational (AcbC, AcbSh), limbic (CeC, VTA) and executive (Cg) system pathways, and may be the primary targets of NMDAR and Fos/Delta fosB that impact morphine withdrawal behaviors. (C) 2016 Published by Elsevier Ireland Ltd.