DNA SUPERCOILING AND THERMAL REGULATION OF UNSATURATED FATTY-ACID SYNTHESIS IN BACILLUS-SUBTILIS
DNA SUPERCOILING AND THERMAL REGULATION OF UNSATURATED FATTY-ACID SYNTHESIS IN BACILLUS-SUBTILIS
复制标题
DOI：
10.1111/j.1365-2958.1994.tb00372.x
复制
发表时间：
1994-03-01
影响因子：
3.6
通讯作者：
DEMENDOZA, D
中科院分区：
文献类型：
--
作者：
GRAU, R;GARDIOL, D;DEMENDOZA, D
在37摄氏度生长的枯草芽孢杆菌几乎完全合成饱和脂肪酸。然而，当在37摄氏度下生长的培养物转移到20摄氏度时，不饱和脂肪酸的合成被诱导。DNA旋转酶抑制剂novobiocin的加入特异性地阻止了20℃下不饱和脂肪酸合成的诱导。此外，从20℃生长的细胞中分离的质粒DNA明显比从37℃生长的细胞中分离的相应DNA具有更多的负超螺旋。总体结果与以下假设一致：随着温度的下降，DNA超螺旋的增加可以调节枯草杆菌中不饱和脂肪酸的合成。
Bacillus subtilis growing at 37 degrees C synthesizes, almost exclusively, saturated fatty acids. However, when a culture growing at 37 degrees C is transferred to 20 degrees C, the synthesis of unsaturated fatty acids is induced. The addition of the DNA gyrase inhibitor novobiocin specifically prevented the induction of unsaturated fatty acid synthesis at 20 degrees C. Furthermore, it was determined that plasmid DNA isolated from cells growing at 20 degrees C was significantly more negatively supercoiled than the equivalent DNA isolated from cells growing at 37 degrees C. The overall results agree with the hypothesis that an increase in DNA supercoiling associated with a temperature downshift could regulate the unsaturated fatty acids synthesis in B. subtilis.