Genes of chicken MHC regulate the adherence activity of blood monocytes in Rous sarcomas progressing and regressing lines
Genes of chicken MHC regulate the adherence activity of blood monocytes in Rous sarcomas progressing and regressing lines
复制标题
DOI：
10.1016/s0165-2427(98)00191-3
复制
发表时间：
1998-11-24
影响因子：
1.8
通讯作者：
Hofmann, A
中科院分区：
文献类型：
--
作者：
Hala, K;Moore, C;Hofmann, A
使用同系鸡系 CB 和 CC 检测鸡主要组织相容性 (B) 复合物 (MHC) 对单核细胞来源的巨噬细胞粘附潜力的影响。这些细胞系代表了明确的遗传模型，用于研究劳斯肉瘤进行性生长的抗性（CB）或敏感性（CC）。使用针对鸡单核细胞/巨噬细胞的单克隆抗体，流式细胞术分析显示 CB 和 CC 鸡具有相似的外周血单核细胞比例。然而，当在 40 摄氏度下在组织培养基中孵育 24、48 和 72 小时期间比较这些细胞的玻璃粘附潜力时，发现这两个近交系的细胞之间存在显着差异。 24和48小时后，CB细胞的玻璃粘附比CC细胞高2-3倍。 72 小时后，这一差异减少至 1.5 倍。 24 和 48 小时时，粘附的 CB 巨噬细胞也比 CC 鸡大约 1.5 倍。使用 FI 杂种 (CBxCC) 进行的遗传分析表明，该性状受显性基因调控，该显性基因与回交代 F(1)xCC 中的 B12 单倍型分离。从重组同系系 CB.R1 和 CC.R1 获得的结果中，我们得出结论，调节粘附潜力的基因位于鸡 MHC 的 B-F/L 区域内。大约 50% 的贴壁细胞能够吞噬经过调理的 FITC 标记的酵母聚糖颗粒。 CB 和 CC 巨噬细胞在体外产生的一氧化氮水平相等。使用封装在脂质体中的抗巨噬细胞剂二氧化硅、角叉菜胶和 Cl(2)MDP，在 CB 鸡中研究了单核吞噬细胞系统细胞对劳斯肉瘤病毒 (RSV) 感染反应的重要性。在那些用二氧化硅和角叉菜胶处理的鸡中，我们观察到 RSV 诱导的肿瘤进行性生长。处理鸡的外周血淋巴细胞（PBL）的移植物抗宿主反应性与对照组相当。体外，经二氧化硅处理的鸡的巨噬细胞产生的一氧化氮高于未经处理的对照鸡的巨噬细胞。 (C) 1998 Elsevier Science B.V. 保留所有权利。
The influence of the chicken major histocompatibility (B) complex (MHC) on the adherence potential of monocyte-derived macrophages was examined using the congenic chicken lines CB and CC. These Lines represent well-defined genetic models for the study of resistance (CB) or susceptibility (CC) to the progressive growth of Rous sarcomas. Using a monoclonal antibody specific for chicken monocytes/macrophages, CB and CC chickens were shown by flow cytometry analyses to have similar proportions of peripheral blood monocytes. However, when the glass-adherence potential of these cells was compared during incubation in tissue culture medium over 24, 48 and 72 h at 40 degrees C, significant differences were seen between cells from these two inbred Lines. After 24 and 48 h, glass-adherence by CB cells was 2-3 fold higher than that of CC cells. After 72 h this difference decreased to 1.5 fold. At 24 and 48 h, the adherent CB macrophages also appeared about 1.5 times larger than those of CC chickens. Genetic analysis using FI hybrids (CBxCC) showed that this trait is regulated by a dominant gene that segregates with the B12 haplotype in the backcross generation F(1)xCC. From the results obtained with the recombinant congenic lines CB.R1 and CC.R1, we conclude that the gene regulating adherence potential is localized within the B-F/L region of the chicken MHC. About 50% of adherent cells were able to phagocytose opsonised FITC-labelled Zymosan particles. The level of nitric oxide production in vitro by CB and CC macrophages was equal. The importance of cells of the mononuclear phagocyte system for the response to Rous sarcoma virus (RSV) infection was studied in CB chickens using the anti-macrophage agents silica, carrageenan, and Cl(2)MDP, encapsulated in liposomes. In those chickens treated with silica and carrageenan, we observed progressive growth of RSV-induced tumors. The graft-versus-host reactivity of peripheral blood lymphocytes (PBL) of treated chickens was comparable to controls. In vitro nitric oxide production by macrophages from silica-treated chickens was higher than by macrophages from untreated controls. (C) 1998 Elsevier Science B.V. All rights reserved.