Curcumin ameliorates methotrexate-induced nephrotoxicity in rats.
Curcumin ameliorates methotrexate-induced nephrotoxicity in rats.
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DOI：
10.1155/2013/387071
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发表时间：
2013
影响因子：
--
通讯作者：
Hassan MK
中科院分区：
文献类型：
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作者：
Morsy MA;Ibrahim SA;Amin EF;Kamel MY;Rifaai RA;Hassan MK
甲氨蝶呤是一种有效的抗癌和免疫抑制药物。然而，肾毒性是使用它的并发症之一。另一方面，姜黄素是一种天然存在的多酚化合物，据报道具有抗氧化和抗炎特性。这两种特性可能会预防甲氨蝶呤引起的肾毒性。本研究旨在评价姜黄素对甲氨蝶呤所致大鼠肾毒性的保护作用，并探讨其可能的作用机制(S)。大鼠连续3d腹腔注射甲氨蝶呤(7 mg/kg/d)，造成肾毒性。甲氨蝶呤中毒大鼠给予姜黄素后，血清肌酐、尿素、肾组织丙二醛、一氧化氮和肿瘤坏死因子-α水平显著降低，而肾组织谷胱甘肽过氧化物酶和超氧化物歧化酶活性显著升高。此外，免疫组织化学分析显示，姜黄素处理显著降低了环氧合酶-2的表达。组织病理学检查证实了姜黄素的保护作用。总而言之，姜黄素保护大鼠免受甲氨蝶呤肾毒性，至少部分是通过其抗氧化和抗炎活性。
Methotrexate is an effective anticancer and immunosuppressive agent. However, nephrotoxicity is one of the complications of its use. On the other hand, curcumin, a naturally occurring polyphenolic compound, is reported to have antioxidant and anti-inflammatory properties. Those two properties are likely to prevent methotrexate-induced nephrotoxicity. The aim of this study is to evaluate the possible protective effect of curcumin against methotrexate-induced nephrotoxicity and delineate various mechanism(s) underlies this effect in rats. Nephrotoxicity was induced in Wistar rats by intraperitoneal administration of methotrexate (7 mg/kg/day) for three consecutive days. Curcumin administration in methotrexate-intoxicated rats resulted in nephroprotective effects as evidenced by the significant decrease in levels of serum creatinine and urea as well as renal malondialdehyde, nitric oxide, and tumor necrosis factor-α with a concurrent increase in renal glutathione peroxidase and superoxide dismutase activities compared to nephrotoxic untreated rats. Additionally, immunohistochemical analysis demonstrated that curcumin treatment markedly reduced cyclooxygenase-2 expression. Histopathological examination confirmed the protective effects of curcumin. In conclusion, curcumin protected rats from methotrexate nephrotoxicity, at least in part, through its antioxidant and anti-inflammatory activities.