Isolation and transcription profiling of purified uncultured human stromal stem cells: Alteration of gene expression after in vitro cell culture
Isolation and transcription profiling of purified uncultured human stromal stem cells: Alteration of gene expression after in vitro cell culture
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DOI：
10.1091/mbc.e04-10-0949
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Brinchmann, JE
中科院分区：
文献类型：
--
作者：
Boquest, AC;Shahdadfar, A;Brinchmann, JE
基质干细胞在体外增殖并可沿沿着几个谱系分化。这些细胞刚分离出来时，数量太少或纯度不够，无法进行彻底的鉴定。在这里，我们已经从人脂肪组织中分离出两个群体的CD 45（-）CD 34（+）CD 105（+）细胞，它们可以根据CD 31的表达进行分离。与CD 31（+）细胞相比，CD 31（-）细胞过度表达与细胞周期静止和干细胞相关的转录本，以及与软骨、骨、脂肪、肌肉和神经组织生物学相关的转录本。相反，CD 31（+）细胞过度表达与内皮和主要组织相容性复合物II类复合物相关的转录本。CD 31（-）细胞克隆在体外可扩增并分化为具有骨、脂肪和神经样组织特征的细胞。在培养中，与细胞周期静止、干细胞、某些细胞因子和器官特异性基因相关的转录物下调，而与信号转导、细胞粘附和细胞骨架成分相关的转录物上调。CD 31（+）细胞在体外不增殖。来自人脂肪组织的CD 45（-）CD 34（+）CD 105（+）CD 31（-）细胞具有基质干细胞的特性，这可能使它们用于组织工程。
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. Freshly isolated, these cells have been too few or insufficiently pure to be thoroughly characterized. Here, we have isolated two populations of CD45(-)CD34(+)CD105(+) cells from human adipose tissue which could be separated based on expression of CD31. Compared with CD31(+) cells, CD31(-) cells overexpressed transcripts associated with cell cycle quiescence and sternness, and transcripts involved in the biology of cartilage, bone, fat, muscle, and neural tissues. In contrast, CD31(+) cells overexpressed transcripts associated with endothelium and the major histocompatibility complex class II complex. Clones of CD31(-) cells could be expanded in vitro and differentiated into cells with characteristics of bone, fat, and neural-like tissue. On culture, transcripts associated with cell cycle quiescence, sternness, certain cytokines and organ specific genes were down-regulated, whereas transcripts associated with signal transduction, cell adhesion, and cytoskeletal components were up-regulated. CD31(+) cells did not proliferate in vitro. CD45(-)CD34(+)CD105(+)CD31(-) cells from human adipose tissue have stromal stem cell properties which may make them useful for tissue engineering.