Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging.
Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging.
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DOI：
10.1021/acs.analchem.1c03935
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发表时间：
2022-02
影响因子：
7.4
通讯作者：
Han Yang;Yayun Wu;Hefei Ruan;F. Guo;Yuxin Liang;Gege Qin;Xiaolong Liu;Zhen Zhang;Jinghe Yuan
中科院分区：
文献类型：
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作者：
Han Yang;Yayun Wu;Hefei Ruan;F. Guo;Yuxin Liang;Gege Qin;Xiaolong Liu;Zhen Zhang;Jinghe Yuan
受激发射耗尽(STED)纳米显微镜是一种新兴的研究细胞结构的超分辨率成像平台。开发尺寸小、光稳定性好、易于功能化的合适的荧光探针仍然是需要的。在这里，我们介绍了一种新型的表面工程金纳米团簇(Au NCs)，它是超小(1.7 nm)和超亮(QY=60%)的，用于STED生物成像。L-精氨酸(L-精氨酸)和6-氮杂-2-硫代胸腺嘧啶(ATT)形成的刚性壳层不仅使其在水溶液中具有较强的荧光，而且使其易于化学修饰以用于特定的生物分子标记。作为STED纳米探针，Au NCs表现出优异的性能，包括高耗尽效率、良好的光漂白性能和低饱和强度。利用这些Au纳米管已经实现了超分辨率成像，并展示了细胞微管蛋白的靶向纳米成像。此外，还揭示了活细胞中溶酶体的环状结构。由于Au-NC也是一种理想的电子显微镜探针，因此在相关光学和电子显微镜(CLEM)中实现了Au-β-42聚集体与Au-Ncs单一标记探针的双重成像。这项工作首次报道了Au NCS作为一种新型探针在STED和CLEM成像中的应用。金纳米管具有优异的性能，在纳米级生物成像方面具有广阔的应用前景。
Stimulated emission depletion (STED) nanoscopy is an emerging super-resolution imaging platform for the study of the cellular structure. Developing suitable fluorescent probes of small size, good photostability, and easy functionalization is still in demand. Herein, we introduce a new type of surface-engineered gold nanoclusters (Au NCs) that are ultrasmall (1.7 nm) and ultrabright (QY = 60%) for STED bioimaging. A rigid shell formed by l-arginine (l-Arg) and 6-aza-2-thiothymine (ATT) on the Au NC surface enables not only its strong fluorescence in aqueous solution but also its easy chemical modification for specific biomolecule labeling. Au NCs show remarkable performance as STED nanoprobes, including high depletion efficiency, good photobleaching resistance, and low saturation intensity. Super-resolution imaging has been achieved with these Au NCs, and targeted nanoscopic imaging of cellular tubulin has been demonstrated. Moreover, the circular structure of lysosomes in live cells has been revealed. As a Au NC is also an ideal probe for electron microscopy, dual imaging of Aβ42 aggregates with the single labeling probe of Au NCs has been realized in correlative light and electron microscopy (CLEM). This work reports, for the first time, the application of Au NCs as a novel probe in STED and CLEM imaging. With their excellent properties, Au NCs show promising potential for nanoscale bioimaging.