Assessment of cell viability in a three-dimensional enzymatically cross-linked collagen scaffold
Assessment of cell viability in a three-dimensional enzymatically cross-linked collagen scaffold
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DOI：
10.1007/s10856-007-3091-9
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发表时间：
2007-10-01
影响因子：
3.7
通讯作者：
Pandit, A.
中科院分区：
文献类型：
--
作者：
Garcia, Y.;Collighan, R.;Pandit, A.
微生物转氨酶（mTGase）是在肽结合的谷氨酰胺和赖氨酸残基之间引入共价键的酶。以这种方式交联的蛋白质通常对蛋白水解降解更具抗性，并显示出增加的拉伸强度。本研究评价了mTGase介导的胶原交联对小鼠3T3成纤维细胞接种到胶原支架中后的细胞形态、行为和活力的影响。此外，使用图像分析软件、扫描电子显微镜（SEM）、比色测定和傅里叶变换红外光谱（FTIR）分析了细胞介导的支架收缩、孔隙率和支架的交联水平。我们证明，生物相容性和细胞形态，当比较培养的成纤维细胞整合在mTGase交联胶原支架与天然胶原对应物，保持不受影响。还已经得出，胶原的结构特征在引入酶抗性共价键的同时得到保留。
Microbial transglutaminase (mTGase) is an enzyme that introduces a covalent bond between peptide bound glutamine and lysine residues. Proteins cross-linked in this manner are often more resistant to proteolytic degradation and show increased tensile strength. This study evaluates the effects of mTGase mediated cross-linking of collagen on the cellular morphology, behaviour and viability of murine 3T3 fibroblasts following their seeding into collagen scaffolds. Additionally, cell mediated scaffold contraction, porosity and level of cross-linking of the scaffold has been analysed using image analysis software, scanning electron microscopy (SEM), colorimetric assays, and Fourier transform infrared spectroscopy (FTIR). We demonstrate that the biocompatibility and cellular morphology, when comparing cultures of fibroblasts integrated in mTGase cross-linked collagen scaffolds with the native collagen counterparts, remained unaffected. It has been also elicited that the structural characteristics of collagen have been preserved while introducing enzymatically resistant covalent bonds.