New in vitro tests to detect the thyroid stimulator in sera from hyperthyroid patients by measuring colloid droplet formation and cyclic AMP in human thyroid slices.
New in vitro tests to detect the thyroid stimulator in sera from hyperthyroid patients by measuring colloid droplet formation and cyclic AMP in human thyroid slices.
复制标题
新的体外测试通过测量人甲状腺切片中的胶滴形成和环磷酸腺苷来检测甲状腺功能亢进患者血清中的甲状腺刺激剂。
DOI：
10.1210/jcem-36-5-859
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
Y. Ochi
中科院分区：
文献类型：
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作者：
T. Onaya;M. Kotani;Takashi Yamada;Y. Ochi
为了检测甲状腺功能亢进患者血清中的甲状腺刺激物质，将人和豚鼠甲状腺切片直接孵育在未经治疗的甲状腺功能亢进患者血清中。在孵育切片的25个卵泡中计数细胞内胶体滴。从未经治疗的甲亢患者中获得的82%的LATS阴性血清确实刺激正常人甲状腺切片中细胞内胶体液滴的形成。需要超过0.1 mU/ml的牛TSH才能产生类似于LATS阴性血清所产生的胶体滴的增加。在正常组织和Graves病的人甲状腺切片中，LATS-IgG也产生了类似的胶体液滴形成增加。正常受试者的血清在这方面没有影响。未经治疗的甲亢患者的血清和LATS-IgG也刺激了体外人甲状腺切片中环AMP的积累。结论LATS-IgG能促进胶质细胞的形成。
In an attempt to detect a thyroidstimulating substance in the sera of hyperthyroid patients, human and guinea pig thyroid slices were incubated directly in the sera of untreated hyperthyroid patients. The intracellular colloid droplets were enumerated in 25 follicles of the incubated slices. Eighty-two % of LATS-negative sera obtained from untreated hyperthyroid patients did stimulate intracellular colloid droplet formation in normal human thyroid slices. More than 0.1 mU/ml of bovine TSH was required to produce the increase of colloid droplets similar to that produced by LATS-negative sera. A similar increase in colloid droplet formation was also produced by LATS-IgG in human thyroid slices of normal tissue and Graves' disease as well. The sera from normal subjects were without effect in this respect. Both the sera from untreated hyperthyroid patients and LATS-IgG also stimulated the accumulation of cyclic AMP in human thyroid slices in vitro. Although LATS-IgG stimulated colloid droplet formation in gui...