Dynamics of CD62L/CD45RB CD4+ and CD8+ lymphocyte subsets in hepatic and splenic tissues during murine visceral leishmaniasis
Dynamics of CD62L/CD45RB CD4+ and CD8+ lymphocyte subsets in hepatic and splenic tissues during murine visceral leishmaniasis
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DOI：
10.1016/j.imlet.2004.06.005
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发表时间：
2004-08-15
影响因子：
4.4
通讯作者：
Santos-Gomes, GM
中科院分区：
文献类型：
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作者：
Gomes-Pereira, S;Rodrigues, OR;Santos-Gomes, GM
本研究首次观察了内脏利什曼病患者CD 4（+）和CD 8（+）淋巴细胞CD 62 L/CD 45 RB亚群的动态变化。比较了婴儿利什曼原虫感染的“治愈”和“未治愈”表型小鼠品系中肝脏和脾脏T细胞的记忆/活化状态。在两种小鼠品系中，检测到肝脏中记忆性CD 4（+）和CD 8（+）T细胞（CD 62 L（低）/CD 45 RB（低））亚群的动态与脾脏淋巴细胞（CD 62 L（低）/CD 45 RB（高））的预活化表型之间的相关性，表明该器官是迁移至肝脏的利什曼原虫特异性T淋巴细胞的来源，其中寄生虫复制高度活跃。在肝脏中，这些预活化的细胞成为效应T淋巴细胞，然而，观察到CD 8（+）T细胞效应功能的强烈调节，可能防止肝组织损伤。比较具有“治愈”和“非治愈”表型的小鼠品系，B10.D2/n动物中“保护性”CD 45 RB（高）和“致病性”CD 45 RB（低）CD 4（+）亚群之间的不平衡可能参与非治愈性感染的演变。(C)2004 Elsevier B. V.保留所有权利。
This study aimed to characterise, for the first time, the dynamics of CD4(+) and CD8(+) lymphocyte CD62L/CD45RB subsets, during visceral leishmaniasis. Memory/activated status of hepatic and splenic T cells was compared in mice strains with "cure" and "non-cure" phenotypes to Leishmania infantum infection. In both mice strains, a correlation between the dynamics of the memory CD4(+) and CD8(+) T cells (CD62L(low)/CD45RB(low)) subsets in the liver and the pre-activated phenotype of lymphocytes (CD62L(low)/CD45RB(high)) from the spleen was detected suggesting that this organ is the source of Leishmania-specific T lymphocytes that migrate to the liver, where parasite replication is highly active. In the liver, these pre-activated cells become effector T lymphocytes, however, a strong regulation of CD8(+) T cell effector function was observed, probably preventing hepatic tissue damage. Comparing mice strains with "cure" and "non-cure" phenotype, an imbalance between "protective" CD45RB(high) and "pathogenic" CD45RB(low) CD4(+) subsets in B10.D2/n animals might be involved in the evolution of a non-healing infection. (C) 2004 Elsevier B.V. All rights reserved.