Expression of Extracellular Matrix Genes During Myocardial Recovery From Heart Failure After Left Ventricular Assist Device Support
Expression of Extracellular Matrix Genes During Myocardial Recovery From Heart Failure After Left Ventricular Assist Device Support
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DOI：
10.1016/j.healun.2008.11.910
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发表时间：
2009-02-01
影响因子：
8.9
通讯作者：
Barton, Paul J. R.
中科院分区：
文献类型：
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作者：
Felkin, Leanne E.;Lara-Pezzi, Enrique;Barton, Paul J. R.
背景：心力衰竭时细胞外基质（ECM）的异常表达。在本研究中，我们分析了晚期扩张型心肌病患者在左心室辅助装置（LVAD）卸载联合药物治疗后是否出现持续心肌恢复的ECM基因表达。心肌胶原基因表达（COL 1A 1和COL 3A 1）、纤连蛋白（FN）、基质金属蛋白酶（MMPs 1至14），金属蛋白酶的组织抑制剂（TIMPs 1至4），结缔组织生长因子（CTGF），转化生长因子β 1在LVAD植入时和再次植入时通过实时逆转录聚合酶链反应（RT-PCR）测量TGF-β 1和THY 1（恢复，n = 11）或移植结果：与恢复组相比，植入时未恢复组的促纤维化标志物（COL 1A 1、TGF-β 1和THY 1）水平更高（分别为1.82 +/- 0.74、1.81 +/- 0.69和3.01 +/- 1.70倍; p
Background: Abnormalities in the extracellular matrix (ECM) can occur in heart failure. in this study we analyzed ECM gene expression in patients with advanced dilated cardiomyopathy who did and did not develop sustained myocardial recovery after left ventricular asst device (LVAD) unloading combined with pharmacologic therapy.Methods: Myocardial gene expression of collagens (COL1A1 and COL3A1), fibronectin (FN), matrix metalloproteinases (MMPs 1 to 14), tissue inhibitors of metalloproteinases (TIMPs 1 to 4), connective tissue growth factor (CTGF), transforming growth factor-betal (TGF-beta 1) and THY1 was measured by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) at LVAD implantation and again at explantation (recovery, n = 11) or transplantation (non-recovery, n = 5).Results: The non-recovery group had higher levels of pro-fibrotic markers (COL1A1, TGF-beta 1 and THY1) at implantation compared with the recovery group (1.82 +/- 0.74-, 1.81 +/- 0.69-, and 3.01 +/- 1.70-fold, respectively; p