Hyperoside and let-7a-5p synergistically inhibits lung cancer cell proliferation via inducing G1/S phase arrest
Hyperoside and let-7a-5p synergistically inhibits lung cancer cell proliferation via inducing G1/S phase arrest
复制标题
金丝桃苷和let-7a-5p通过诱导G1/S期阻滞协同抑制肺癌细胞增殖
DOI：
10.1016/j.gene.2018.09.011
复制
发表时间：
2018-12-30
期刊：
影响因子：
3.5
通讯作者：
Zhang, Tong-Cun
中科院分区：
文献类型：
--
作者：
Li, Jia-Peng;Liao, Xing-Hua;Zhang, Tong-Cun
肺癌仍然是最具侵袭性的人类恶性肿瘤之一，生存率低。金丝桃苷（quercetin 3-O-beta-D-galactopyranoside）是具有抗癌活性的黄酮醇糖苷。microRNA-let-7被广泛认为是人类肿瘤中的肿瘤抑制因子。本研究探讨金丝桃苷和let-7a-5 p对体外培养的肺癌A549细胞增殖、细胞周期和凋亡的影响。结果表明金丝桃苷可通过诱导A549细胞凋亡和G1/S期阻滞抑制A549细胞增殖。Let-7a-5 p可通过抑制G1/S期进程抑制A549细胞增殖。此外，金丝桃苷和let-7a-5 p对A549细胞增殖的抑制作用具有协同作用，microRNA-let-7a-5 p直接调控A549细胞中CCND 1的表达。本研究表明金丝桃苷与microRNA-let 7a-5 p可能具有协同抗肿瘤作用，为肺癌的治疗提供了新的思路。
Lung cancer remains one of the most aggressive human malignancies with a low survival rate. Hyperoside (quercetin 3-O-beta-D-galactopyranoside) is a flavonol glycoside with an anti-cancer activity. The microRNA-let-7 was widely regarded as a tumor suppressor in human tumors. Here, we investigated the role of hyperoside and let-7a-5p on the lung cancer cell proliferation, cell cycle and apoptosis in A549 cells in vitro. Our results showed that hyperoside could inhibit the proliferation of A549 cells through inducing apoptosis and G1/S phase arrest. Let-7a-5p could inhibit the proliferation of A549 cells via inhibiting the process of G1/S phase. Additionally, hyperoside and let-7a-5p had a synergetic effect on suppressing the proliferation of A549 cells; microRNA-let-7a-5p directly regulated the expression of CCND1 in A549 cells. Our study illustrated that hyperoside and microRNA-let7a-5p might provide a synergistic effect on anti-cancer, which may provide a new idea for lung cancer treatment.