Evidence for neuropeptide Y synthesis in the rat anterior pituitary and the influence of thyroid hormone status: comparison with vasoactive intestinal peptide, substance P, and neurotensin.
Evidence for neuropeptide Y synthesis in the rat anterior pituitary and the influence of thyroid hormone status: comparison with vasoactive intestinal peptide, substance P, and neurotensin.
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大鼠垂体前叶神经肽 Y 合成和甲状腺激素状态影响的证据：与血管活性肠肽、P 物质和神经降压素的比较。
DOI：
10.1210/endo-125-1-334
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发表时间：
1989
期刊：
影响因子：
4.8
通讯作者：
S. Bloom
中科院分区：
文献类型：
--
作者：
P. Jones;M. Ghatei;J. Steel;D. O'Halloran;G. Gon;S. Legon;J. Burrin;U. Leonhardt;J. Polak;S. Bloom
神经肽Y（neuropeptide Y，NPY）是胰多肽家族的一个成员，它由36个氨基酸组成。通过北方印迹分析，在垂体中鉴定了NPY激素原信使RNA（mRNA）。通过确定甲状腺激素操纵对肽合成的影响来检查NPY的可能调节。其他三种垂体前叶神经肽，神经降压素（NT），P物质（SP）和血管活性肠肽（VIP），进行了比较研究。甲减组垂体内NPY、SP、VIP及其mRNA含量显著增加，NT含量显著减少。免疫细胞化学显示，在对照组大鼠垂体前叶散在的细胞非常弱的NPY免疫反应，但在甲状腺功能减退大鼠的阳性细胞数量较多，染色相对较强。这些阳性细胞被鉴定为促甲状腺细胞的一个子集。在T4诱导的甲状腺功能亢进症，神经肽Y，NT和VIP水平不受影响，而SP浓度下降相当大。TRH治疗引起NT降低，对NPY、SP或VIP无影响。这些变化仅在垂体中发现;下丘脑肽和mRNA水平没有发生净变化。由于垂体肽和mRNA水平的变化协调发生，因此甲状腺激素状态的调节至少部分直接在基因转录水平上发生。这4种调节肽在甲状腺功能减退症中的变化及其对垂体功能的强大作用表明，它们可能在控制垂体分泌的改变中具有显著的旁分泌或自分泌影响。
Neuropeptide Y (NPY), a 36-amino acid member of the pancreatic polypeptide family, was found to be present by RIA and immunocytochemistry in the rat anterior pituitary gland. NPY prohormone messenger RNA (mRNA) was identified in the pituitary by Northern blot analysis. The possible regulation of NPY was examined by determining the effects of thyroid hormone manipulation on peptide synthesis. Three other anterior pituitary neuropeptides, neurotensin (NT), substance P (SP), and vasoactive intestinal peptide (VIP), were studied for comparison. Hypothyroidism was found to significantly increase the pituitary content of NPY, SP, and VIP and their respective mRNAs but to decrease the quantity of NT. Immunocytochemistry revealed very weak NPY immunoreactivity in scattered cells in control rat anterior pituitaries, but in hypothyroid rats a greater number of positive cells were seen, and the staining was relatively intense. These positive cells were identified as a subset of thyrotropes. In T4-induced hyperthyroidism NPY, NT, and VIP levels were unaffected whereas SP concentrations fell considerably. TRH treatment produced a decrease in NT and had no effect on NPY, SP, or VIP. These changes were found only in the pituitary; no net change occurred in hypothalamic peptide and mRNA levels. Since the changes in pituitary peptide and mRNA levels occurred coordinately it appears that regulation by thyroid hormone status occurs, at least in part, directly at the level of gene transcription. The changes in these 4 regulatory peptides in hypothyroidism and their known powerful effects on pituitary function suggest that they may have a significant paracrine or autocrine influence in controlling the alterations in pituitary secretion.
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甲状腺激素对大鼠垂体前叶 P 物质浓度的调节。
影响因子：
4.8
作者：
Aronin,N;Morency,K;Leeman,SE;Braverman,LE;Coslovsky,R
通讯作者：
Coslovsky,R
血管活性肠肽是大鼠催乳素释放的生理介质。
影响因子：
4.8
作者：
Abe,H;Engler,D;Molitch,ME;Bollinger-Gruber,J;Reichlin,S
通讯作者：
Reichlin,S
甲状腺激素状态调节雄性大鼠垂体前叶前原速激肽-A 基因表达。
影响因子：
4.8
作者：
Jonassen,JA;Mullikin-Kilpatrick,D;McAdam,A;Leeman,SE
通讯作者：
Leeman,SE
有证据表明，释放到垂体-门静脉循环中的神经肽 Y (NPY) 参与促性腺激素释放激素 (GnRH) 的作用。
影响因子：
4.8
作者：
Sutton,SW;Toyama,TT;Otto,S;Plotsky,PM
通讯作者：
Plotsky,PM
DOI：
10.1016/s0021-9258(18)68881-7
发表时间：
1988-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
E. Kislauskis;B. Bullock;S. McNeil;P. Dobner
通讯作者：
E. Kislauskis;B. Bullock;S. McNeil;P. Dobner