Molecular Cloning and Characterization of a Linalool Synthase from Lemon Myrtle
Molecular Cloning and Characterization of a Linalool Synthase from Lemon Myrtle
复制标题
柠檬香桃木中芳樟醇合酶的分子克隆和表征
DOI：
10.1271/bbb.100922
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
H. Sakai
中科院分区：
文献类型：
--
作者：
Mizue Sugiura;S. Ito;Yosuke Saito;Y. Niwa;A. Koltunow;O. Sugimoto;H. Sakai
使用同源性为基础的PCR策略，我们确定了一个cDNA的序列相似性芳樟醇合成酶从柠檬桃金娘。cDNA（命名为BcLS）基因在大肠杆菌中的功能性表达产生了能够催化香叶基二磷酸转化为（-）-芳樟醇的活性酶，即，无环单萜醇，以及更少量的环状单萜。BcLS的动力学参数与环单萜类化合物生成酶的动力学参数相似。PCR分析表明，BcLS基因转录普遍表达在柠檬桃金娘和上调响应茉莉酸处理。尽管BcLS依赖于橙花基二磷酸（NPP）的生理作用尚不清楚，但当NPP用作底物时，BcLS的环化活性增强，导致主要产生环状单萜。这些发现表明BcLS具有新的特异性和动力学参数，但其对诸如昆虫损伤的应激的生理反应似乎与已知的芳樟醇脱氢酶相似。
Using a homology-based PCR strategy, we identified a cDNA with sequence similarity to linalool synthase from lemon myrtle. Functional expression of the cDNA (designated BcLS) gene in Escherichia coli yielded an active enzyme capable of catalyzing the conversion of geranyl diphosphate to (−)-linalool, i.e., an acyclic monoterpene alcohol, and to lesser amounts of cyclic monoterpenes. The kinetic parameters of BcLS were similar to those of synthases producing cyclic monoterpenes. PCR analysis revealed that the BcLS gene transcript was ubiquitously expressed in lemon myrtle and was upregulated in response to jasmonic acid treatment. Although the physiological role of neryl diphosphate (NPP) dependency of BcLS remains unclear, the cyclization activity of BcLS was enhanced when NPP was used as substrate, resulting in predominant production of cyclic monoterpenes. These findings indicate that BcLS has novel specificity and kinetic parameters, but its physiological responses to stresses such as insect damage appear to be similar to known linalool synthases.
影响因子：
3.9
作者：
Crowell, AL;Williams, DC;Croteau, R
通讯作者：
Croteau, R
影响因子：
2.9
作者：
Williams, DC;McGarvey, DJ;Croteau, R
通讯作者：
Croteau, R