CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage
CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage
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DOI：
10.1093/emboj/16.6.1360
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发表时间：
1997-03-17
期刊：
影响因子：
11.4
通讯作者：
Kappes, DJ
中科院分区：
文献类型：
--
作者：
Dave, VP;Cao, ZS;Kappes, DJ
与T细胞受体(TCR)相关的CD3复合体在TCR参与后的信号转导中是必不可少的。在T细胞发育过程中，TCR介导的信号传递促进了从一个发育阶段到下一个发育阶段的过渡，并控制胸腺细胞是经历正选择还是负选择，但特定的CD3成分在这些事件中的作用尚不清楚。事实上，目前还不清楚他们是有专门的角色还是有重叠的角色。然而，CD3组分的多样性及其进化保守性表明，它们具有不同的功能。在这里，通过产生一个专门缺乏CD3增量链的小鼠系(Delta-/-小鼠)来分析CD3增量链的发育需求，引人注目的是，CD3增量被证明在发育过程中是不同的需要。特别是，CD3 Delta不是由前TCR或Gamma Delta TCR介导的发育步骤所必需的，而是表达α-βTCR的胸腺细胞进一步发育所必需的，CD3 Delta的缺失特别地阻止了胸腺选择过程，该过程介导了从双阳性到单阳性发育阶段的转变。
The CD3 complex found associated with the T cell receptor (TCR) is essential for signal transduction following TCR engagement. During T cell development, TCR-mediated signalling promotes the transition from one developmental stage to the next and controls whether a thymocyte undergoes positive or negative selection, The roles of particular CD3 components in these events remain unclear. Indeed, it is unknown whether they have specialized or overlapping roles. However, the multiplicity of CD3 components and their evolutionary conservation suggest that they serve distinct functions. Here the developmental requirement for the CD3 delta chain is analyzed by generating a mouse line specifically lacking this component (delta-/- mice), Strikingly, CD3 delta is shown to be differentially required during development. In particular, CD3 delta is not needed for steps in development mediated by pre-TCR or gamma delta TCR, but is required for further development of thymocytes expressing alpha beta TCR, Absence of CD3 delta specifically blocks the thymic selection processes that mediate the transition from the double-positive to single-positive stages of development.