THE REELER GENE ENCODES A PROTEIN WITH AN EGF-LIKE MOTIF EXPRESSED BY PIONEER NEURONS
THE REELER GENE ENCODES A PROTEIN WITH AN EGF-LIKE MOTIF EXPRESSED BY PIONEER NEURONS
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DOI：
10.1038/ng0595-77
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发表时间：
1995-05-01
期刊：
影响因子：
30.8
通讯作者：
HAYASHIZAKI, Y
中科院分区：
文献类型：
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作者：
HIROTSUNE, S;TAKAHARA, T;HAYASHIZAKI, Y
我们已经鉴定出小鼠 reeler 基因的一个强有力的候选 cDNA。这个 5 kb 转录物编码一个 99.4 kD 的蛋白质，由 881 个氨基酸组成，并具有两个 EGF 样基序。我们检测了两个独立的突变等位基因——“Jackson reeler”，它具有整个基因的缺失，以及“Orleans reeler”，它在开放阅读框中显示出 220 bp 的缺失，包括第二个 EGF 样基序并导致移码。原位杂交表明，转录物仅在引导神经元细胞沿径向阵列迁移的先驱神经元中检测到。我们的研究结果解释了 reeler 突变表型如何导致神经元网络复杂结构的紊乱。
We have identified a strong candidate cDNA for the mouse reeler gene. This 5 kb transcript encodes a 99.4 kD protein consisting of 881 amino acids and possessing two EGF-like motifs. We assayed two independent mutant alleles - 'Jackson reeler', which has a deletion of the entire gene, and 'Orleans reeler' which exhibits a 220 bp deletion in the open reading frame, including the second EGF-like motif and resulting in a frame shift. In situ hybridization reveals that the transcript is detected exclusively in the pioneer neurons which guide neuronal cell migration along the radial array. Our findings offer an explanation for how the reeler mutant phenotype causes a disturbance of the complex architecture of the neuronal network.