Cryptochromes and phytochromes synergistically regulate Arabidopsis root greening under blue light.
Cryptochromes and phytochromes synergistically regulate Arabidopsis root greening under blue light.
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DOI：
10.1093/pcp/pch205
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发表时间：
2004-12
影响因子：
4.9
通讯作者：
T. Usami;N. Mochizuki;M. Kondo;M. Nishimura;A. Nagatani
中科院分区：
文献类型：
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作者：
T. Usami;N. Mochizuki;M. Kondo;M. Nishimura;A. Nagatani
为了增加它们的适应性，植物通过利用多种光感受器（例如光敏色素、隐花色素和趋光素）来感知环境光条件并调节它们的发育过程。即使是根，通常不暴露在光下，表达光受体，并可以通过发展叶绿体对光作出反应。在本研究中，根绿化观察拟南芥。幼苗在单色光下生长，并测定根中的叶绿素水平。结果发现，蓝光是更有效地诱导叶绿体发育在拟南芥根比红光，这种反应的控制下，一个强大的协同作用光敏色素和隐花色素。正如预期的那样，cry1突变体在这种反应中存在缺陷。有趣的是，phyAphyB双突变体在这些条件下对蓝光没有反应。这强烈表明，光敏色素A或光敏色素B，除了隐花色素，是需要这种蓝光响应。进一步证明了光合基因的表达也以同样的方式受到调控。双色照射实验表明，这种相互作用依赖于phyB P（FR）的水平。对cop1、det1和hy5突变体的分析表明，相应的因子参与了应答。
To increase their fitness, plants sense ambient light conditions and modulate their developmental processes by utilizing multiple photoreceptors such as phytochrome, cryptochrome and phototropin. Even roots, which are normally not exposed to light, express photoreceptors and can respond to light by developing chloroplasts. In the present study, root greening was observed in Arabidopsis thaliana. Seedlings were grown under monochromatic light and chlorophyll levels in the roots were determined. It was found that blue light was far more effective at inducing chloroplast development in Arabidopsis roots than was red light, and this response was under the control of a strong synergistic interaction between phytochromes and cryptochromes. As expected, the cry1 mutant was deficient in this response. Interestingly, the phyAphyB double mutant failed to respond to blue light under these conditions. This strongly suggests that either phytochrome A or phytochrome B, in addition to cryptochrome, was required for this blue light response. It was further demonstrated that the expression of photosynthetic genes was regulated in the same way. Dichromatic irradiation experiments indicated that this interaction depends on the level of phyB P(FR). Analysis of the cop1, det1 and hy5 mutants indicated that the corresponding factors were involved in the response.