NEW LYMPHOCYTE ANTIGEN SYSTEM (LNA) CONTROLLED BY IR-REGION OF MOUSE H-2 COMPLEX
NEW LYMPHOCYTE ANTIGEN SYSTEM (LNA) CONTROLLED BY IR-REGION OF MOUSE H-2 COMPLEX
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DOI：
10.1073/pnas.70.9.2509
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发表时间：
1973-01-01
影响因子：
11.1
通讯作者：
FRELINGER, JA
中科院分区：
文献类型：
--
作者：
DAVID, CS;SHREFFLER, DC;FRELINGER, JA
本文描述了一种新的小鼠淋巴细胞同种抗原系统。这种淋巴结抗原系统与H-2（组织相容性-2）基因复合体的Ir区有关。它具有以下特点：（1）控制这些抗原的基因定位在H-2K和Ss-Slp之间的免疫反应区。(2)这些抗原在淋巴结细胞上最容易检测到，尽管它们也在外周血淋巴细胞、脾淋巴细胞和胸腺细胞上表达。(3)始终观察到仅对约一半淋巴结细胞的细胞毒性。(4)针对这些抗原的细胞毒性抗体滴度惊人地高-通过51 Cr释放超过2000，并且在微量细胞毒性试验中高达100，000。(5)至少有两种可能是等位基因形式的抗原已被确定，一种与H-2k单倍型有关，另一种与H-2a单倍型有关。(6)抗L的抗血清含有多种抗体特异性，可通过与某些重组体或具有交叉反应性抗原的其他H-2卤代型的吸收而分离。抗Lna的抗血清对于定义和表征与H-2复合物相关的IrandMLR（混合淋巴细胞反应刺激）基因的产物可能具有价值。
A new system of lymphocyte alloantigens in mice is described. ThisLna(lymph-node antigen) system is associated with theIrregion of theH-2(histocompatibility-2) gene complex. It has the following distinctive characteristics: (1) The gene or genes controlling these antigens has been mapped in theIr(immune response) region betweenH-2KandSs-Slp. (2) The antigens are most readily detectable on lymph-node cells, although they are also expressed on peripheral blood lymphocytes, splenic lymphocytes, and thymocytes. (3) Cytotoxicity against only about half of lymph-node cells is consistently observed. (4) Cytotoxic antibody titers against these antigens are strikingly high—more than 2000 by51Cr-release and up to 100,000 in the microcytotoxic test. (5) At least two, probably allelic, forms of the antigen(s) have been defined, one associated with theH-2khaplotype and one with theH-2ahaplotype. (6) Antisera againstLnacontain multiple antibody specificities that can be fractionated by absorption either with certain recombinants or with otherH-2halotypes that have crossreactive antigens. The antisera againstLnamay be of value for definition and characterization of the products of theIrandMLR(mixed lymphocyte reaction stimulatory) genes associated with theH-2complex.