Eccentric exercise increases circulating fibroblast activation protein a but not bioactive fibroblast growth factor 21 in healthy humans.
Eccentric exercise increases circulating fibroblast activation protein a but not bioactive fibroblast growth factor 21 in healthy humans.
复制标题
在健康人中，离心运动会增加循环成纤维细胞活化蛋白 a，但不会增加生物活性成纤维细胞生长因子 21。
DOI：
10.1113/ep086669
复制
发表时间：
2018
影响因子：
2.7
通讯作者：
Parmar B
中科院分区：
文献类型：
--
作者：
Parmar B
新发现本研究的中心问题是什么？FGF 21作为运动诱导的肌因子的作用仍然存在争议。本研究的目的是确定离心运动是否会增加FGF 21和/或其调节酶成纤维细胞活化蛋白α（FAP）从骨骼肌组织释放到健康志愿者的体循环中。离心运动不会从人骨骼肌中释放总的或生物活性的FGF 21。然而，运动可从肌肉以外的组织释放其调节酶FAP，这可能在FGF 21的失活中起作用。本研究的主要目的是确定离心运动是否会增加肌因子成纤维细胞生长因子21（FGF 21）和/或其调节酶成纤维细胞活化蛋白α（FAP）的释放，从骨骼肌组织进入健康志愿者的体循环。身体活跃的年轻健康男性志愿者（年龄25.0 ± 10.7岁;体重指数23.1 ± 7.9 kg m−2）使用非优势腿完成了三组25次重复（中间休息5分钟）的单腿最大离心收缩，而优势腿作为对照。动脉化血液样品从手静脉和深静脉血液样品从股总静脉的行使腿，沿着与股浅动脉的血流使用多普勒超声，之前和之后的每一个运动回合和每20分钟在3小时的恢复期。在基线、运动后即刻、运动后3 h和48 h采集肌肉活检样本。主要发现表明，在运动反应中，从骨骼肌分泌到体循环中的总FGF 21或生物活性FGF 21没有显著增加。此外，骨骼肌FGF 21蛋白含量在运动中没有变化。然而，有一个显着增加动脉和静脉FAP浓度，没有明显的贡献，其从运动腿的释放。这些发现提高了FAP水平升高可能在运动期间FGF 21失活中发挥作用的可能性。
New FindingsWhat is the central question of this study?The role of FGF21 as an exercise‐induced myokine remains controversial. The aim of this study was to determine whether eccentric exercise would augment the release of FGF21 and/or its regulatory enzyme, fibroblast activation protein α (FAP), from skeletal muscle tissue into the systemic circulation of healthy human volunteers.What is the main finding and its importance?Eccentric exercise does not release total or bioactive FGF21 from human skeletal muscle. However, exercise releases its regulatory enzyme, FAP, from tissue(s) other than muscle, which might play a role in the inactivation of FGF21.AbstractThe primary aim of the investigation was to determine whether eccentric exercise would augment the release of the myokine fibroblast growth factor 21 (FGF21) and/or its regulatory enzyme, fibroblast activation protein α (FAP), from skeletal muscle tissue into the systemic circulation of healthy human volunteers. Physically active young healthy male volunteers (age 25.0 ± 10.7 years; body mass index 23.1 ± 7.9 kg m−2) completed three sets of 25 repetitions (with 5 min rest in between) of single‐leg maximal eccentric contractions using their non‐dominant leg, whilst the dominant leg served as a control. Arterialized blood samples from a hand vein and deep venous blood samples from the common femoral vein of the exercised leg, along with blood flow of the superficial femoral artery using Doppler ultrasound, were obtained before and after each exercise bout and every 20 min during the 3 h recovery period. Muscle biopsy samples were taken at baseline, immediately and 3 and 48 h postexercise. The main findings showed that there was no significant increase in total or bioactive FGF21 secreted from skeletal muscle into the systemic circulation in response to exercise. Furthermore, skeletal muscle FGF21 protein content was unchanged in response to exercise. However, there was a significant increase in arterialized and venous FAP concentrations, with no apparent contribution to its release from the exercised leg. These findings raise the possibility that the elevated levels of FAP might play a role in the inactivation of FGF21 during exercise.