An allelic series for studying the mouse Thoc1 gene
An allelic series for studying the mouse Thoc1 gene
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DOI：
10.1002/dvg.20262
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发表时间：
2007-01
期刊：
影响因子：
1.5
通讯作者：
Xiaoling Wang;Yanping Li;Xiaojing Zhang;D. Goodrich
中科院分区：
文献类型：
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作者：
Xiaoling Wang;Yanping Li;Xiaojing Zhang;D. Goodrich
TCR 1编码哺乳动物TREX蛋白复合物的必需组分。TREX是一种进化上保守的复合物，它将延长RNA聚合酶II与RNA加工因子偶联。TCR 1蛋白（pTCR 1）的缺失损害了一些RNA的转录延伸和核输出。TGF 1的缺失导致小鼠围着床期胚胎死亡。早期胚胎致死性排除了在发育中的胚胎或成体中对THB 1的生理需求的分析。为了规避这一限制，我们已经产生了小鼠含有亚纯型或条件等位基因的TAP 1。条件等位基因纯合子小鼠表现正常。含有Cre重组条件等位基因的小鼠表型复制先前表征的Tcl 1无效等位基因。亚型等位基因纯合子的小鼠是可存活的，并且以与预期的孟德尔比率没有显著差异的频率出生。然而，这些小鼠比野生型小鼠表达更少的pTGF 1，并表现出侏儒表型。侏儒表型可以在妊娠中期胚胎中检测到，这表明在胚胎和出生后发育后期也需要TGF 1。创世纪44：32-37，2007。2007年出版Wiley利斯公司
Thoc1 encodes an essential component of the mammalian TREX protein complex. TREX is an evolutionary conserved complex that couples elongating RNA polymerase II with RNA processing factors. Depletion of Thoc1 protein (pThoc1) compromises transcriptional elongation and nuclear export of some RNAs. Loss of Thoc1 causes periimplantation embryonic lethality in the mouse. Early embryonic lethality precludes analysis of the physiological requirements for Thoc1 in the developing embryo or adult. To circumvent this limitation, we have generated mice containing hypomorphic or conditional alleles of Thoc1. Mice homozygous for the conditional allele appear normal. Mice containing Cre recombined conditional alleles phenocopy the previously characterized Thoc1 null allele. Mice homozygous for the hypomorphic allele are viable and born at a frequency that is not significantly different from the expected Mendelian ratio. However, these mice express less pThoc1 than wild type mice and exhibit a dwarf phenotype. The dwarf phenotype can be detected in mid‐gestation embryos, suggesting that Thoc1 is also required later in embryonic and postnatal development. genesis 44:32–37, 2007. Published 2007 Wiley‐Liss, Inc.