Repetitive mechanical stretch increases extracellular collagenase activity in vaginal fibroblasts.
Repetitive mechanical stretch increases extracellular collagenase activity in vaginal fibroblasts.
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DOI：
10.1097/spv.0b013e3181ed30d2
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发表时间：
2010-09-01
影响因子：
1.6
通讯作者：
Moalli PA
中科院分区：
文献类型：
--
作者：
Zong W;Jallah ZC;Stein SE;Abramowitch SD;Moalli PA
目的是1）确定人阴道成纤维细胞是否是机械敏感的，2）研究在激素存在和不存在的情况下机械拉伸对这些细胞的影响。从3名妇女的全层阴道活检中获得的成纤维细胞在8和16%的幅度下周期性双轴拉伸72小时，有或没有17-β-雌二醇和孕酮。收集培养基，并使用荧光底物降解试验一式两份测量总胶原酶活性。使用Student t检验在0.05显著性水平下分析数据。在整个实验过程中，细胞保持90%的存活率。相对于对照组，单独激素治疗降低胶原酶活性（P=0.008）。在存在机械拉伸和不存在激素的情况下，胶原酶活性增加（分别为8%伸长率，P=0.04; 16%伸长率，P=0.001）。胶原酶活性的增加与幅度呈线性相关（P<0.001）。在激素的存在下，酶活性的机械拉伸的增加被抑制到基线对照水平（P=0.46）。激素的抑制程度无差异（P=0.48）。阴道结缔组织成纤维细胞是机械敏感的，在拉伸的存在下胶原酶活性增加。这种降解行为在激素的存在下受到抑制。这些数据提供了一种机制，通过这种机制，诱导阴道牵拉的事件可能导致盆腔器官脱垂的进展，特别是在缺乏激素的情况下。需要进一步的研究来确定这些事件是否导致组织具有较差的机械性能。
The objectives were 1) to determine whether human vaginal fibroblasts are mechanosensitive and 2) to study the impact of mechanical stretch on these cells in the presence and absence of hormones. Fibroblasts obtained from biopsies of full thickness vagina of 3 women were cyclically biaxially stretched at a magnitude of 8 and 16% for 72 hours with or without 17-β-estradiol plus progesterone. Culture media was collected and total collagenase activity was measured in duplicate using a fluorogenic substrate degradation assay. Data were analyzed at the 0.05 level of significance using Student t-test. Cells remained 90% viable throughout the experiments. Relative to the controls, hormonal treatment alone decreased collagenase activity (P=0.008). In the presence of mechanical stretch and in the absence of hormones, collagenase activity was increased (8% elongation, P=0.04; 16% elongation, P=0.001, respectively). The increase in collagenase activity was linearly correlated with magnitude (P<0.001). In the presence of hormones, the increase in enzyme activity by mechanical stretch was suppressed to baseline control levels (P=0.46). There was no difference in suppression by hormones by magnitude (P=0.48). Vaginal connective tissue fibroblasts are mechanosensitive with increased collagenase activity in the presence of stretch. This degradative behavior is inhibited in the presence of hormones. The data provide a mechanism by which events that induce vaginal stretch may lead to progression of pelvic organ prolapse, particularly, in the absence of hormones. Further studies are needed to determine whether these events lead to tissue with inferior mechanical properties.