Remote Floating-Gate Field-Effect Transistor with 2-Dimensional Reduced Graphene Oxide Sensing Layer for Reliable Detection of SARS-CoV-2 Spike Proteins
Remote Floating-Gate Field-Effect Transistor with 2-Dimensional Reduced Graphene Oxide Sensing Layer for Reliable Detection of SARS-CoV-2 Spike Proteins
复制标题
具有二维还原氧化石墨烯传感层的远程浮栅场效应晶体管，用于可靠检测 SARS-CoV-2 刺突蛋白
DOI：
10.1021/acsami.2c04969
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发表时间：
2022
影响因子：
9.5
通讯作者：
Ankenbruck, Nicholas
中科院分区：
文献类型：
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作者：
Jang, Hyun-June;Sui, Xiaoyu;Zhuang, Wen;Huang, Xiaodan;Chen, Min;Cai, Xiaolei;Wang, Yale;Ryu, Byunghoon;Pu, Haihui;Ankenbruck, Nicholas
尽管基于纳米材料的场效应晶体管（FET）作为一种快速诊断工具进行了深入的研究，但由于缺乏稳定性、可靠性、可重复性和大规模生产的可扩展性，FET传感器用于临床应用仍有待观察。在此，我们提出了一种远程浮栅（RFG）场效应管配置，以消除二维还原氧化石墨烯（rGO）传感表面的器件间变化和溶液界面上的大部分不稳定性。此外，通过对还原氧化石墨烯厚度、覆盖范围和还原温度变化的还原氧化石墨烯溶液界面的广泛研究，确定了还原氧化石墨烯电化学不稳定性背后的关键机制因素，如严重漂移和滞后。我们的RFGFET结构中的氧化石墨烯表面显示出54 mV/pH（从pH 2到11）的Nernstian响应，产率为90%（总共10个样品中的9个），变异系数(CV) < 3%，低漂移率为2%，所有这些都是由绝对测量值计算得出的。作为概念验证，我们证明了在唾液相关培养基中高度可靠、可重复和无标记地检测严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）刺突蛋白，浓度范围为500 fg/mL至5 μg/mL， anr2值为0.984,CV < 3%，保证检测限为几pg/mL。总而言之，这个新平台可能对在临床环境中定位FET生物电子学以检测SARS-CoV-2产生巨大影响。
Despite intensive research of nanomaterials-based field-effect transistors (FETs) as a rapid diagnostic tool, it remains to be seen for FET sensors to be used for clinical applications due to a lack of stability, reliability, reproducibility, and scalability for mass production. Herein, we propose a remote floating-gate (RFG) FET configuration to eliminate device-to-device variations of two-dimensional reduced graphene oxide (rGO) sensing surfaces and most of the instability at the solution interface. Also, critical mechanistic factors behind the electrochemical instability of rGO such as severe drift and hysteresis were identified through extensive studies on rGO–solution interfaces varied by rGO thickness, coverage, and reduction temperature. rGO surfaces in our RFGFET structure displayed a Nernstian response of 54 mV/pH (from pH 2 to 11) with a 90% yield (9 samples out of total 10), coefficient of variation (CV) < 3%, and a low drift rate of 2%, all of which were calculated from the absolute measurement values. As proof-of-concept, we demonstrated highly reliable, reproducible, and label-free detection of spike proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a saliva-relevant media with concentrations ranging from 500 fg/mL to 5 μg/mL, with anR2value of 0.984 and CV < 3%, and a guaranteed limit of detection at a few pg/mL. Taken together, this new platform may have an immense effect on positioning FET bioelectronics in a clinical setting for detecting SARS-CoV-2.