Temperature-dependent phase behavior and protein partitioning in giant plasma membrane vesicles.
Temperature-dependent phase behavior and protein partitioning in giant plasma membrane vesicles.
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DOI：
10.1016/j.bbamem.2010.03.009
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发表时间：
2010-07
影响因子：
3.4
通讯作者：
Baumgart, T.
中科院分区：
文献类型：
--
作者：
Johnson, S. A.;Stinson, B. M.;Go, M. S.;Carmona, L. M.;Reminick, J. I.;Fang, X.;Baumgart, T.
关键词：
已经提出了液体有序（Lo）和液体无序（Ld）相共存以将生物细胞的质膜划分为横向隔室，从而允许功能相关分子的富集或耗尽。这种动态分配可能涉及通过耦合到质膜蛋白和脂质组合物微调细胞信号保真度。在早期的工作中，观察到通过化学诱导培养细胞起泡获得的巨大质膜囊泡在显着低于37 °C的温度下可逆地相分离。在这方面的贡献，我们比较了HeLa和大鼠嗜碱性白血病（RBL）细胞的流体相分离的温度依赖性。我们发现一个基本上单调的温度依赖性的相分离的囊泡在这两种细胞类型的数量。我们还观察到一个惊人的广泛分布的相变温度在这两种细胞类型。外周蛋白，如霍乱毒素亚基B（CT B），以及膜联蛋白V的结合，观察到调节相变温度，表明外周蛋白结合可能是一个调节器的横向异质性在体内。许多信号蛋白锚和全长蛋白质的分区进行了研究。我们发现Lo相分区的几种蛋白质在文献中假设是膜筏相关，但观察到偏离这一预期的其他蛋白质，包括小窝蛋白-1。
Liquid-ordered (Lo) and liquid-disordered (Ld) phase coexistence has been suggested to partition the plasma membrane of biological cells into lateral compartments, allowing for enrichment or depletion of functionally relevant molecules. This dynamic partitioning might be involved in fine-tuning cellular signaling fidelity through coupling to the plasma membrane protein and lipid composition. In earlier work, giant plasma membrane vesicles, obtained by chemically induced blebbing from cultured cells, were observed to reversibly phase segregate at temperatures significantly below 37 °C. In this contribution, we compare the temperature dependence of fluid phase segregation in HeLa and Rat basophilic leukemia (RBL) cells. We find an essentially monotonic temperature dependence of the number of phase separated vesicles in both cell types. We also observe a strikingly broad distribution of phase transition temperatures in both cell types. The binding of peripheral proteins, such as cholera toxin subunit B (CTB), as well as annexin V, is observed to modulate phase transition temperatures, indicating that peripheral protein binding may be a regulator for lateral heterogeneity in vivo. The partitioning of numerous signal protein anchors and full length proteins is investigated. We find Lo phase partitioning for several proteins assumed in the literature to be membrane raft associated, but observe deviations from this expectation for other proteins, including caveolin-1.
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影响因子：
3.4
作者：
Frolov, V. A. J.;Chizmadzhev, Y. A.;Zimmerberg, J.
通讯作者：
Zimmerberg, J.
影响因子：
3.4
作者：
Feigenson, GW;Buboltz, JT
通讯作者：
Buboltz, JT
影响因子：
3.4
作者：
Dietrich, C;Bagatolli, LA;Gratton, E
通讯作者：
Gratton, E
DOI：
10.1073/pnas.92.20.9201
发表时间：
1995-09-26
影响因子：
11.1
作者：
FIELD, KA;HOLOWKA, D;BAIRD, B
通讯作者：
BAIRD, B
影响因子：
16.6
作者：
Bauer, Brigitte;Davidson, Max;Orwar, Owe
通讯作者：
Orwar, Owe