Response of gabaergic cells in the deep mesencephalic nucleus to dopaminergic cell degeneration:: An electrophysiological and in situ hybridization study
Response of gabaergic cells in the deep mesencephalic nucleus to dopaminergic cell degeneration:: An electrophysiological and in situ hybridization study
复制标题
DOI：
10.1016/s0306-4522(02)00186-0
复制
发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Rodríguez, M
中科院分区：
文献类型：
--
作者：
González-Hernández, T;Barroso-Chinea, P;Rodríguez, M
中脑深核(DMN)是位于黑质致密区和上丘之间的一个较大的中脑网状区。它含有GABA能细胞，它们与黑质有相同的纹状体传入、丘脑和丘脑传出，以及神经化学和电生理相似之处。本文用电生理(放电频率和放电模式)和形态(谷氨酸脱羧酶(GAD)65和GAD67mRNA原位杂交组织化学标记的密度分析)技术，研究了DMN GABA能细胞对黑质多巴胺能细胞变性的反应。结果表明，单侧前脑内侧束注射6-羟基多巴胺后，单侧多巴胺能细胞丢失导致双侧对称性放电频率和GAD67基因表达增加，GAD65基因表达降低。这些发现支持DMN GABA能细胞参与多巴胺能细胞丢失后的基底节改变，也表明它参与了帕金森病的病理生理学。作用的对称性，以及最近报道的对丘脑和上丘的双侧投射，表明DMN不同于黑质网状质，参与了基底节的半球间调节，即使在不对称损伤后，可能仍保持其功能对称性。(C)2002年IBRO。爱思唯尔科学有限公司出版。版权所有。
The deep mesencephalic nucleus (DMN) is a large midbrain reticular region located between the substantia nigra compacta and the superior colliculus. It contains GABAergic cells that share striatal afferents, thalamic and collicular efferents, as well as neurochemical and electrophysiological similarities, with those of the substantia nigra reticulata. In the present paper we used electrophysiological (firing rate and firing pattern) and morphological (densitometric analysis of in situ hybridization histochemical labeling for glutamic acid decarboxylase (GAD)65 and GAD67 mRNA) techniques, to study the response of DMN GABAergic cells to the degeneration of nigral dopaminergic cells. Our results showed that unilateral dopaminergic cell loss (after injection of 6-hydroxydopamine in the medial forebrain bundle) induces a bilateral and symmetrical increase in both firing rate and GAD67 mRNA levels and a decrease in GAD65 mRNA levels. These findings support the involvement of DMN GABAergic cells in the basal ganglia modifications that follow dopaminergic cell loss, also suggesting its participation in the pathophysiology of Parkinson's disease.The symmetry of effects, together with its recently reported bilateral projections to the thalamus and superior colliculus, suggest that unlike substantia nigra reticulata, DMN is involved in the interhemispheric regulation of basal ganglia, probably keeping their functional symmetry even after asymmetric lesions. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.