Effect of relaxin on TGF-β1 expression in cultured vaginal fibroblasts from women with stress urinary incontinence
Effect of relaxin on TGF-β1 expression in cultured vaginal fibroblasts from women with stress urinary incontinence
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DOI：
10.1177/1933719108315299
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发表时间：
2008-03-01
影响因子：
2.9
通讯作者：
Chen, Bertha
中科院分区：
文献类型：
--
作者：
Wen, Yan;Zhao, Yang-Yu;Chen, Bertha
本研究的目的是比较松弛素对压力性尿失禁（SUI）女性和大陆女性（对照组）在月经周期两个阶段的阴道成纤维细胞中转化生长因子（TGF）-β 1和潜伏性TGF-β 1结合蛋白（LTBP-1）的影响。用松弛素处理成纤维细胞。TGF-β 1水平通过酶联免疫吸附试验测定。通过Western blot检测LTBP-1的表达。在增殖期，SUI成纤维细胞的上清液、细胞和细胞外基质（ECM）中的总TGF-β 1水平随着松弛素浓度的增加而降低（P <0.05）。在SUI成纤维细胞培养上清中，低浓度松弛素可使活性TGF-β 1水平升高（P <0.05），而高浓度松弛素则使细胞外基质中活性TGF-β 1水平降低（P <0.05）。在分泌期，SUI患者和对照组的上清液、细胞和ECM中的总TGF-β 1水平随着松弛素治疗而降低（P <0.05）。松弛素降低了从SUI阴道成纤维细胞分离的ECM中总的和活性TGF-β 1的水平。
The objective of this study is to compare relaxin's effect on transforming growth factor (TGF)-beta 1 and latent TGF-beta 1-binding protein (LTBP-1) in vaginal fibroblasts from women with stress urinary incontinence (SUI) to continent women (controls) in both phases of the menstrual cycle. Fibroblasts were treated with relaxin. TGF-beta 1 levels were measured by enzyme-linked immunosorbent assay. LTBP-1 expression was evaluated by Western blot. In the proliferative phase, total TGF-beta 1 level in the supernatant, cells, and extracellular matrix (ECM) of SUI fibroblasts decreased with increasing relaxin concentration (P < .05). Active TGF-beta 1 levels increased at a low concentration Of relaxin (P < .05) in the supernatant but decreased in the ECM of SUI fibroblasts at high concentration (P < .05). In the secretory phase, total TGF-beta 1 levels decreased with relaxin treatment (P < .05) in the supernatant, cells and ECM of both women with SUI and controls. Relaxin. decreased the levels of total and active TGF-beta 1 in the ECM isolated from SUI vaginal fibroblasts.