Pseudomonas syringae pv. avii (pv. nov.), the causal agent of bacterial canker of wild cherries (Prunus avium) in France
Pseudomonas syringae pv. avii (pv. nov.), the causal agent of bacterial canker of wild cherries (Prunus avium) in France
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DOI：
10.1023/a:1024786201793
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发表时间：
2003-07-01
影响因子：
1.8
通讯作者：
Gardan, L
中科院分区：
文献类型：
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作者：
Ménard, M;Sutra, L;Gardan, L
采用生化数值分类、DNA-DNA杂交、基于REP、ERIC和BOX序列的重复序列引物pcr （REP - pcr）、内部转录间隔物（ITS）异双工迁移率测定（HMA）以及对野生樱桃树和其他李属植物的致病性等方法，对从法国野生樱桃树（Prunus avium）溃疡病中分离的菌株进行了鉴定。将它们与从野生樱桃、甜樱桃和各种寄主植物中分离的丁香假单胞菌病原菌株进行比较。在LOPAT组la（+ - - - +）中，野樱桃菌株与丁香P （sensu lato）亲缘关系较近。野生樱桃菌株对野生樱桃树致病，产生的症状与在果园中观察到的相似。它们对甜樱桃树也有致病作用，但程度较轻。拿破仑)。野生樱桃菌株是从法国五个不同的地区收集的，似乎构成了一个非常同质的群体。它们在生化和生理特征上表现出相同的特征。经DNA-DNA杂交鉴定，两者亲缘关系较近，属3‘番茄’基因组种。Rep-PCR结果表明，野生樱桃菌株构成了一个与p.s.v v不同的紧密类群。morsprunorum小种1和2以及来自其他丁香科病原菌。HMA图谱显示，所有野生樱桃品系的ITS基本相同，但与P.s. pv不同。由于这两条迁移率降低的异双工带是通过与p.s.v v杂交而产生的。桃科病原菌CFBP 1573。Gardan et al.（1999）的8个基因组物种还没有转化为正式物种，因为它们无法通过生化测试进行区分。因此，目前使用的是丁香属内的病原系统。丁香P。avii被认为是引起野樱桃溃疡病的细菌，菌株CFBP 3846 （NCPPB 4290, ICMP 14479）被指定为致病型。
Bacterial strains isolated from cankers of wild cherry trees (Prunus avium) in France were characterized using numerical taxonomy of biochemical tests, DNA-DNA hybridization, repeat sequence primed-PCR (rep-PCR) based on REP, ERIC and BOX sequences, heteroduplex mobility assay (HMA) of internal transcribed spacer (ITS) as well as pathogenicity on wild cherry trees and other species of Prunus. They were compared to reference strains of Pseudomonas syringae pathovars isolated from wild and sweet cherry and various host plants. Wild cherry strains were closely related to P syringae (sensu lato) in LOPAT group la (+ - - - +). Wild cherry strains were pathogenic to wild cherry trees and produced symptoms similar to those observed in orchards. They were pathogenic also, but at a lesser extent, to sweet cherry trees (cv. Napoleon). The wild cherry strains were collected from five different areas in France and appeared to constitute a very homogeneous group. They showed an homogenous profile of a biochemical and physiological characteristics. They were closely related by DNA-DNA hybridization and belonged to genomospecies 3 'tomato'. Rep-PCR showed that wild cherry strains constitute a tight group distinct from P.s. pv. morsprunorum races 1 and 2 and from other P syringae pathovars. HMA profiles indicated that the ITS of all wild cherry strains were identical but different from P.s. pv. persicae strains since the two heteroduplex bands with reduced mobility were generated by hybridization with the P.s. pv. persicae pathotype strain CFBP 1573. The 8 genomospecies of Gardan et al. (1999) have not been converted into formal species as they cannot be differentiated by biochemical tests. Therefore, the pathovar system within P syringae was currently used. P syringae pv. avii is proposed for this bacterium causing a wild cherry bacterial canker and strain CFBP 3846 (NCPPB 4290, ICMP 14479) is designated as the pathotype.