The Drosophila homolog of Aut1 is essential for autophagy and development
The Drosophila homolog of Aut1 is essential for autophagy and development
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DOI：
10.1016/s0014-5793(03)00431-9
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发表时间：
2003-05-22
期刊：
影响因子：
3.5
通讯作者：
Sass, M
中科院分区：
文献类型：
--
作者：
Juhász, G;Csikós, G;Sass, M
果蝇同源酵母Aut 1，CG 6877/Draut 1，是一种普遍表达的胞质蛋白。Draut 1功能丧失是通过表达反向重复转基因诱导RNA干扰实现的。该效应是温度依赖性的，并且类似于如Fortier，E.和Belote，J.M.（创世纪26（2000）240-244）。Draut 1功能丧失幼虫在化蛹前不能诱导脂肪体细胞的自噬和异噬，在变态期死亡。据我们所知，这是第一个报告的多细胞动物缺乏功能的基因参与蛋白质结合系统的自噬。(C)2003年由Elsevier Science B. V.代表欧洲生物化学学会联合会出版。
The Drosophila homolog of yeast Aut1, CG6877/Draut1, is a ubiquitously expressed cytosolic protein. Draut1 loss of function was achieved by expression of an inverted repeat transgene inducing RNA interference. The effect is temperature-dependent and resembles an allelic series as described by Fortier, E. and Belote, J.M. (Genesis 26 (2000) 240-244). Draut1 loss of function larvae are unable to induce autophagy and heterophagy in fat body cells before pupariation and die during metamorphosis. To our knowledge, this is the first report of a multicellular animal lacking the function of a gene participating in the protein conjugation systems of autophagy. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.