Endomorphin-2 is not released from rat spinal dorsal horn in response to intraplantar formalin.
Endomorphin-2 is not released from rat spinal dorsal horn in response to intraplantar formalin.
复制标题
Endomorphin-2 不会因足底内福尔马林的反应而从大鼠脊髓背角释放。
DOI：
10.1016/s0304-3940(02)00998-9
复制
发表时间：
2002
影响因子：
2.5
通讯作者：
Dun,NaeJ
中科院分区：
文献类型：
--
作者：
Williams,CaroleA;Ricketts,BrianA;Hua,Fang;Dun,NaeJ
将抗体包被的微探针插入L4-5水平的脊髓中，用于检测是否响应于注射到后爪足垫中的福尔马林而从麻醉大鼠的脊髓背角释放内吗啡肽-2（Endo 2）。生理盐水注射液作为对照，测量P物质（SP）以验证伤害性传入纤维的激活。在预刺激期间释放SP，但不释放Endo 2。生理盐水注射不会导致脊髓释放Endo 2或SP。福尔马林注射引起Fos表达增加以及SP的释放，但不是从同侧L4-5背角的Endo 2。我们的结论是，Endo 2不发挥作用，在体内介导急性炎症伤害性信号在脊髓水平在麻醉大鼠模型的反应。
Antibody coated microprobes, inserted into the spinal cord at the L4–5 level, were used to detect whether endomorphin-2 (Endo2) was released from spinal dorsal horns in anesthetized rats in response to formalin injected into the hindpaw footpads. Saline injections were used as a control and substance P (SP) was measured to verify activation of nociceptive afferent fibers. SP but not Endo2 was released during pre-stimulation periods. Saline injections did not cause the release of either Endo2 or SP from the spinal cord. Formalin injections caused an increase in Fos expression as well as a release of SP, but not Endo2 from the ipsilateral side dorsal horn in L4–5. We conclude that Endo2 does not play a role in mediating the in vivo responses to acute inflammatory nociceptive signals at the spinal level in the anesthetized rat model.