Occurrence, distribution, and role in abortion of Coxiella burnetii in sheep and goats in Sardinia, Italy
Occurrence, distribution, and role in abortion of Coxiella burnetii in sheep and goats in Sardinia, Italy
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DOI：
10.1016/j.vetmic.2004.01.006
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发表时间：
2004-04-19
影响因子：
3.3
通讯作者：
Tola, S
中科院分区：
文献类型：
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作者：
Masala, G;Porcu, R;Tola, S
在1999年和2002年之间，9349血清和517流产样本（422个胎儿和95胎盘）进行了分析，从675只绵羊和82个山羊农场分布在整个撒丁岛。流产通知后，通过ELISA检查从成年动物随机采集的血清，以检测贝氏柯克斯体特异性抗体，而通过PCR测定分析胎儿和胎盘。特异性IgG抗体检测255（38%）绵羊农场和39（47%）山羊畜群，而40绵羊（10%）和3（6%）山羊胎儿C贝氏PCR阳性。虽然C.从不同组织中扩增贝氏体DNA，胎盘组织检出率最高。血清阳性率分析表明，C。贝氏锥虫在绵羊和山羊中的分布率很高，但PCR结果表明，贝氏锥虫在绵羊和山羊中的分布率很高。伯内特氏菌在流产中的作用相对较低，特别是在山羊中。(C)2004 Elsevier B.V.保留所有权利。
Between 1999 and 2002, 9349 sera and 517 aborted samples (422 foetuses and 95 placenta) were analysed from 675 sheep and 82 goat farms distributed all over the island of Sardinia. After abortion notification, sera collected at random from adult animals were examined to detect antibodies specific to Coxiella burnetii by ELISA, whereas foetuses and placenta were analysed by PCR assay. Specific IgG antibodies were detected in 255 (38%) sheep farms and in 39 (47%) goat herds whereas 40 ovine (10%) and 3 (6%) caprine foetuses were C burnetii PCR-positive. Although C. burnetii DNA was amplified from different types of tissues, placenta was the tissue with the highest detection rate. Seroprevalence analysis indicates that C. burnetii distribution in sheep and goats is very high, but PCR results demonstrate that C. burnetii has a relatively low role in abortion, especially in goats. (C) 2004 Elsevier B.V. All rights reserved.