HIF-1α-induced autophagy contributes to cisplatin resistance in ovarian cancer cells
HIF-1α-induced autophagy contributes to cisplatin resistance in ovarian cancer cells
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DOI：
10.1691/ph.2018.8514
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发表时间：
2018-09-01
期刊：
影响因子：
1.6
通讯作者：
Wang, Ting
中科院分区：
文献类型：
--
作者：
Long, Fangyi;Liu, Weixin;Wang, Ting
本研究采用MTT法和流式细胞仪检测卵巢癌细胞株OVCAR-3的存活率和凋亡情况。GFP标记的LC 3质粒转染用于证明自噬的发生。Western blot检测HIF-1 α、Beclin 1、LC 3和β-actin的表达。我们发现缺氧可以抑制顺铂诱导的OVCAR-3细胞凋亡，并增强顺铂的耐药性。此外，GFP标记的LC 3质粒转染和蛋白质印迹用于证明缺氧诱导的OVCAR-3细胞对顺铂的化学抗性与HIF-1 α诱导的自噬有关。所有这些发现表明卵巢癌细胞的顺铂耐药性与HIF-1 α诱导的自噬有关。
In this study, the viability and apoptosis of ovarian cancer cell line OVCAR-3 were assessed using MTT and flow cytometry analysis. GFP-tagged LC3 plasmid transfection was utilized to demonstrate the occurrence of autophagy. The expression of HIF-1 alpha, Beclin1, LC3 and beta-actin were determined with Western blot analysis. We found that hypoxia could inhibit cisplatin induced apoptosis in OVCAR-3 cells and enhance the chemoresistance to cisplatin. Furthermore, GFP-tagged LC3 plasmid transfection and western blot were used to demonstrate that hypoxia induced chemoresistance of OVCAR-3 cells to cisplatin is related to HIF-1 alpha-induced autophagy. All these findings suggest that the cisplatin resistance of ovarian cancer cells is associated with HIF-1 alpha-induced autophagy.