The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn.
The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn.
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DOI：
10.1016/j.cell.2016.12.010
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发表时间：
2017-01-12
期刊：
影响因子：
64.5
通讯作者：
Ginty DD
中科院分区：
文献类型：
--
作者：
Abraira VE;Kuehn ED;Chirila AM;Springel MW;Toliver AA;Zimmerman AL;Orefice LL;Boyle KA;Bai L;Song BJ;Bashista KA;O'Neill TG;Zhuo J;Tsan C;Hoynoski J;Rutlin M;Kus L;Niederkofler V;Watanabe M;Dymecki SM;Nelson SB;Heintz N;Hughes DI;Ginty DD
深背角是一个缺乏特征性的脊髓区域，涉及处理低阈值机械感受器（LTMR）信息。我们报告了一系列小鼠遗传工具，用于定义背角LTMR受体区（LTMR-RZ）的神经元成分和功能，LTMR-RZ处理在触觉感知中的作用，以及LTMR-RZ组织的基本逻辑。我们发现了一个出乎意料的高度的神经元多样性的LTMR-RZ：7兴奋性和4抑制亚型的interneurons表现出独特的形态，生理和突触特性。值得注意的是，LTMR在4至11个LTMR-RZ中间神经元亚型上形成突触，而每个LTMR-RZ中间神经元亚型对来自至少1至3个LTMR类别以及脊髓中间神经元和皮质脊髓神经元的输入进行采样。因此，LTMR-RZ是一个躯体感觉处理区域，具有与视网膜相竞争的神经元复杂性，并用于对触觉感知基础的上行触摸通路的活动进行模式化。会聚的LTMR和皮层输入定义了机械感觉背角背角分子遗传工具箱定义了11种中间神经元亚型背角中间神经元接收皮层和LTMR输入的特定模式背角中间神经元调节输出通路和触觉感知背角的蓝图揭示了触觉感知所必需的低阈值机械感受器受体区的组织逻辑。
The deep dorsal horn is a poorly characterized spinal cord region implicated in processing low-threshold mechanoreceptor (LTMR) information. We report an array of mouse genetic tools for defining neuronal components and functions of the dorsal horn LTMR-recipient zone (LTMR-RZ), a role for LTMR-RZ processing in tactile perception, and the basic logic of LTMR-RZ organization. We found an unexpectedly high degree of neuronal diversity in the LTMR-RZ: seven excitatory and four inhibitory subtypes of interneurons exhibiting unique morphological, physiological, and synaptic properties. Remarkably, LTMRs form synapses on between four and 11 LTMR-RZ interneuron subtypes, while each LTMR-RZ interneuron subtype samples inputs from at least one to three LTMR classes, as well as spinal cord interneurons and corticospinal neurons. Thus, the LTMR-RZ is a somatosensory processing region endowed with a neuronal complexity that rivals the retina and functions to pattern the activity of ascending touch pathways that underlie tactile perception. Convergent LTMR and cortical inputs define the mechanosensory dorsal horn A dorsal horn molecular-genetic toolbox defines 11 interneuron subtypes Dorsal horn interneurons receive specific patterns of cortical and LTMR inputs Dorsal horn interneurons modulate output pathways and tactile perception The blueprint of dorsal horn reveals the organizational logic of the low-threshold mechanoreceptor-recipient zone that is essential for tactile perception.