The genome of Bacillus tequilensis EA-CB0015 sheds light into its epiphytic lifestyle and potential as a biocontrol agent.
The genome of Bacillus tequilensis EA-CB0015 sheds light into its epiphytic lifestyle and potential as a biocontrol agent.
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DOI：
10.3389/fmicb.2023.1135487
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发表时间：
2023
影响因子：
5.2
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中科院分区：
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不同种类的芽孢杆菌已被成功地用作生物杀虫剂，以防治广泛的植物病原体。其中，龙舌兰芽孢杆菌EA-CB0015已被证明能够有效地控制香蕉植株上的黑斑病，可能是通过改变叶圈环境的适应机制来实现的。在这里，我们报道了菌株EA-CB0015的完整基因组，其准确的分类鉴定，并确定了可能有助于其有效生防植物病原菌的关键遗传特征。我们发现B.tequilensis EA-CB0015含有一个单一的4 Mb环状染色体，有3,951个蛋白质编码序列。多位点序列分析(MLSA)和平均核苷酸同源性(ANI)分析将菌株EA-CB0015归类为B.tequilsis。在其基因组中编码的是生物合成基因簇(BGC)，包括表面蛋白、iturin、plipastatin、杆菌素、杆菌素、亚硫菌素A、产孢素杀伤因子和其他可能促进微生物间战争的天然产物。吲哚-乙酸(IAA)合成的基因，对不同碳源的利用，以及涉及运动性、生物膜形成、群体感应、能力和产孢量的多细胞生活方式，表明EA-CB0015擅长在植物表面定殖。在入侵的病毒感染中存活并保持基因组完整性的防御机制包括假定的I型和II型限制性内切酶修饰(RM)和毒素/抗毒素(TA)系统。噬菌体序列、基因组岛、转座元件、毒力因子和抗生素耐药基因的存在表明先前发生过遗传交换。总之，EA-CB0015的基因组支持其作为生防剂对抗植物病原体的功能，并表明它已经适应了在叶圈环境中茁壮成长。
Different Bacillus species have successfully been used as biopesticides against a broad range of plant pathogens. Among these, Bacillus tequilensis EA-CB0015 has shown to efficiently control Black sigatoka disease in banana plants, presumably by mechanisms of adaptation that involve modifying the phyllosphere environment. Here, we report the complete genome of strain EA-CB0015, its precise taxonomic identity, and determined key genetic features that may contribute to its effective biocontrol of plant pathogens. We found that B. tequilensis EA-CB0015 harbors a singular 4 Mb circular chromosome, with 3,951 protein-coding sequences. Multi-locus sequence analysis (MLSA) and average nucleotide identity (ANI) analysis classified strain EA-CB0015 as B. tequilensis. Encoded within its genome are biosynthetic gene clusters (BGCs) for surfactin, iturin, plipastatin, bacillibactin, bacilysin, subtilosin A, sporulation killing factor, and other natural products that may facilitate inter-microbial warfare. Genes for indole-acetic acid (IAA) synthesis, the use of diverse carbon sources, and a multicellular lifestyle involving motility, biofilm formation, quorum sensing, competence, and sporulation suggest EA-CB0015 is adept at colonizing plant surfaces. Defensive mechanisms to survive invading viral infections and preserve genome integrity include putative type I and type II restriction modification (RM) and toxin/antitoxin (TA) systems. The presence of bacteriophage sequences, genomic islands, transposable elements, virulence factors, and antibiotic resistance genes indicate prior occurrences of genetic exchange. Altogether, the genome of EA-CB0015 supports its function as a biocontrol agent against phytopathogens and suggest it has adapted to thrive within phyllosphere environments.