X-ray damage in protein-metal hybrid structures: a photoemission electron microscopy study.
X-ray damage in protein-metal hybrid structures: a photoemission electron microscopy study.
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蛋白质-金属杂化结构中的 X 射线损伤：光电发射电子显微镜研究
DOI：
10.1021/jp1040585
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
S.L. Molodtsov
中科院分区：
文献类型：
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作者：
K. Kummer;D.V. Vyalikh;S. Danzenbächer;A. Blüher;M. Mertig;A. Lanzara;A. Scholl;S.L. Molodtsov
用光电子显微镜进行侧向分辨的近边X射线吸收精细结构谱研究了细菌表面层蛋白质片（S层）上的钴或银膜。与在原始S层上获得的结果进行比较，使我们能够表征这些蛋白质-金属杂化系统中的化学相互作用和X射线损伤。特别是，我们发现，除了暴露于X射线辐射时的直接损伤外，生物分子还经历了沉积金属的额外贡献，即金属颗粒中的低能电子产生。
Bacterial surface layer protein sheets (S layer) coated with an ultrathin cobalt or silver film were studied by means of laterally resolved near-edge X-ray absorption fine structure spectroscopy performed by photoemission electron microscopy. Comparison with results obtained on pristine S layers allowed us to characterize both chemical interaction and X-ray damage in these protein−metal hybrid systems. In particular, we found that besides direct damage upon exposure to X-ray radiation the biomolecules experience additional contribution of the deposited metals, by low-energy electron generation in the metal particles.