Visualizing mechanical tension across membrane receptors with a fluorescent sensor
Visualizing mechanical tension across membrane receptors with a fluorescent sensor
复制标题
DOI：
10.1038/nmeth.1747
复制
发表时间：
2012-01-01
期刊：
影响因子：
48
通讯作者：
Salaita, Khalid S.
中科院分区：
文献类型：
--
作者：
Stabley, Daniel R.;Jurchenko, Carol;Salaita, Khalid S.
我们报告了一种基于荧光的开启传感器，用于绘制活细胞中特定细胞表面蛋白质施加的机械应变。该传感器使用传统的荧光显微镜生成具有高空间和时间分辨率的力图。我们证明了映射机械力的配体激活的表皮生长因子受体（EGFR）的早期阶段的监管内吞作用的方法。
We report a fluorescence-based turn-on sensor for mapping the mechanical strain exerted by specific cell-surface proteins in living cells. The sensor generates force maps with high spatial and temporal resolution using conventional fluorescence microscopy. We demonstrate the approach by mapping mechanical forces during the early stages of regulatory endocytosis of the ligand-activated epidermal growth factor receptor (EGFR).