Growth-related changes in specific mRNAs of cultured mouse cells.
Growth-related changes in specific mRNAs of cultured mouse cells.
复制标题
培养小鼠细胞特定 mRNA 的生长相关变化。
DOI：
10.1073/pnas.80.14.4271
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发表时间：
1983
影响因子：
11.1
通讯作者：
Nathans,D
中科院分区：
文献类型：
--
作者：
Linzer,DI;Nathans,D
利用血清刺激后BALB/c3T3细胞中的poly(A)+ RNA构建cDNA质粒文库。在测试的3500个克隆中，大约0.5%含有插入物，这些插入物对应于血清刺激的BALB/c 3T3细胞培养物中比静止培养物中存在更高水平的mrna。这些RNA种类大多增加了2- 5倍，并且各种RNA的增加动力学不同。一个克隆（28H6）与一种1千碱基RNA杂交，这种RNA在静息细胞中几乎检测不到，但在血清刺激后增加至少15- 20倍，在DNA合成开始时达到最大水平。这种RNA在增殖细胞中处于高水平，但在细胞到达汇合时迅速下降。28H6 RNA在感染猿猴病毒40或受血小板衍生生长因子刺激的静息细胞中也增加。
A cDNA plasmid library has been constructed from the poly(A)+ RNA present in BALB/c3T3 cells after serum stimulation. Of 3,500 clones tested, approximately 0.5% contained inserts corresponding to mRNAs present at higher levels in serum-stimulated BALB/c 3T3 cell cultures than in quiescent cultures. Most of these RNA species increased 2- to 5-fold, and the kinetics of increase for various RNAs differed. One clone (28H6) hybridized to a 1-kilobase RNA species that is present at barely detectable levels in resting cells but is increased at least 15- to 20-fold after serum stimulation, reaching a maximal level coincident with the onset of DNA synthesis. This RNA was at a high level in proliferating cells but decreased rapidly as cells reached confluence. 28H6 RNA was also increased in resting cells infected with simian virus 40 or stimulated with platelet-derived growth factor.