An archaebacterial homologue of the essential eubacterial cell division protein FtsZ
An archaebacterial homologue of the essential eubacterial cell division protein FtsZ
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DOI：
10.1073/pnas.93.13.6726
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发表时间：
1996-06-25
影响因子：
11.1
通讯作者：
Jackson, SP
中科院分区：
文献类型：
--
作者：
Baumann, P;Jackson, SP
生命福尔斯可分为三个基本领域--藻类、细菌和真核生物虽然古细菌缺乏细胞核，并且与细菌有许多共同的形态特征，但分子分析，主要是转录和翻译机制，表明古细菌与真核生物的关系比细菌更近，目前，对古细菌细胞分裂装置知之甚少，在细菌中，细胞分裂机制的关键组分是FtsZ，一种定位于分隔位点处的环的GTP酶。有趣的是，FtsZ在序列上与真核微管蛋白远亲，真核微管蛋白也与GTP相互作用并且是真核细胞细胞骨架的组分。通过筛选结合放射性标记的核苷酸的能力，我们已经确定了一种蛋白质的超嗜热古菌Pyrococcus woesei紧密和特异性地与GTP相互作用。通过筛选沃氏疟原虫基因组DNA表达文库，克隆了编码该蛋白的基因。序列比较显示，沃氏毕赤酵母GTP结合蛋白在序列上与真细菌FtsZ显著相关，并且与真核微管蛋白的相似性略高于细菌FtsZ蛋白。系统发育分析加强了FtsZ和微管蛋白之间存在进化联系的国家。这些发现表明古细菌的细胞分裂装置可能与细菌的细胞分裂装置基本相似，并使我们考虑古细菌，细菌和真核生物之间的进化关系。
Life falls into three fundamental domains-Archaea, Bacteria, and Eucarya (formerly archaebacteria, eubacteria, and eukaryotes, respectively), Though Archaea lack nuclei and share many morphological features with Bacteria, molecular analyses, principally of the transcription and translation machineries, have suggested that Archaea are more related to Eucarya than to Bacteria, Currently, little is known about the archaeal cell division apparatus, In Bacteria, a crucial component of the cell division machinery is FtsZ, a GTPase that localizes to a ring at the site of septation, Interestingly, FtsZ is distantly related in sequence to eukaryotic tubulins, which also interact with GTP and are components of the eukaryotic cell cytoskeleton. By screening for the ability to bind radiolabeled nucleotides, we have identified a protein of the hyperthermophilic archaeon Pyrococcus woesei that interacts tightly and specifically with GTP. Furthermore, through screening an expression library of P. woesei genomic DNA, we have cloned the gene encoding this protein. Sequence comparisons reveal that the P. woesei GTP-binding protein is strikingly related in sequence to eubacterial FtsZ and is marginally more similar to eukaryotic tubulins than are bacterial FtsZ proteins. Phylogenetic analyses reinforce the nation that there is an evolutionary linkage between FtsZ and tubulins. These findings suggest that the archaeal cell division apparatus may be fundamentally similar to that of Bacteria and lead us to consider the evolutionary relationships between Archaea, Bacteria, and Eucarya.