Activation of dorsal development by contact between the cortical dorsal determinant and the equatorial core cytoplasm in eggs of Xenopus laevis.
Activation of dorsal development by contact between the cortical dorsal determinant and the equatorial core cytoplasm in eggs of Xenopus laevis.
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非洲爪蟾卵中皮质背侧决定簇与赤道核心细胞质接触激活背侧发育。
作者：
H. Kageura
在非洲爪哇的卵中，受精后，通过皮质旋转在未来的背侧激活背部发育。大脑皮层旋转的直接影响可能是将背侧的决定因素从植物极点输送到未来背侧的赤道区。然而，背侧决定因素的身份和作用仍然存在问题。在本实验中，从未受精卵和胚胎的不同区域分离的单个皮质被植入受体8-细胞胚胎的几个位置中的一个。次生轴的出现不仅被用来在不同的时间定位皮质背侧决定因素，而且还被用来定位核心有能力对背侧决定因素做出反应的区域。皮层的背轴诱导活动发生在未受精卵的营养极周围。在皮质旋转的过程中，它从那里转移到一个宽阔的背侧区域。这显然是卵子皮质中存在背侧决定因素的第一个证据。8-细胞胚心的感受性呈梯度分布，赤道响应性最强。这些结果表明，在正常胚胎中，背侧发育是通过皮质背侧决定簇与赤道核心细胞质之间的接触而激活的，这些细胞质通过皮质旋转而聚集在一起。
In eggs of Xenopus laevis, dorsal development is activated on the future dorsal side by cortical rotation, after fertilization. The immediate effect of cortical rotation is probably the transport of a dorsal determinant from the vegetal pole to the equatorial region on the future dorsal side. However, the identity and action of the dorsal determinant remain problematic. In the present experiments, individual isolated cortices from various regions of the unfertilized eggs and embryos were implanted into one of several positions of a recipient 8-cell embryo. The incidence of secondary axes was used not only to locate the cortical dorsal determinant at different times but also to locate the region of the core competent to respond to the dorsal determinant. The dorsal axis-inducing activity of the cortex occurred around the vegetal pole of the unfertilized egg. During cortical rotation, it shifted from there to a wide dorsal region. This is apparently the first evidence for the presence of a dorsal determinant in the egg cortex. The competence of the core of the 8-cell embryo was distributed in the form of gradient with the highest responsiveness at the equator. These results suggest that, in the normal embryo, dorsal development is activated by contact between the cortical dorsal determinant and the equatorial core cytoplasm, brought together through cortical rotation.
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两种植物定位 RNA Vg1 和 Xcat-2 的定位模式和细胞骨架关联。
DOI：
10.1242/dev.121.1.201
发表时间：
1995
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Forristall,C;Pondel,M;Chen,L;King,ML
通讯作者：
King,ML
活爪蟾卵的共聚焦显微镜分析和皮层旋转机制。
DOI：
10.1242/dev.122.4.1281
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Larabell,CA;Rowning,BA;Wells,J;Wu,M;Gerhart,JC
通讯作者：
Gerhart,JC
非洲爪蟾卵的皮质旋转：胚胎背侧发育前后模式的后果。
DOI：
10.1242/dev.107.supplement.37
发表时间：
1989
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Gerhart,J;Danilchik,M;Doniach,T;Roberts,S;Rowning,B;Stewart,R
通讯作者：
Stewart,R
DOI：
10.1073/pnas.94.4.1224
发表时间：
1997-02-18
影响因子：
11.1
作者：
Rowning, BA;Wells, J;Larabell, CA
通讯作者：
Larabell, CA