Spread of levan-positive populations of Pseudomonas savastanoi pv. savastanoi, the causal agent of olive knot, in central Italy
Spread of levan-positive populations of Pseudomonas savastanoi pv. savastanoi, the causal agent of olive knot, in central Italy
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DOI：
10.1007/s10658-005-0804-0
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发表时间：
2005-06-01
影响因子：
1.8
通讯作者：
Surico, G
中科院分区：
文献类型：
--
作者：
Marchi, G;Viti, C;Surico, G
萨瓦斯坦假单胞菌致病变种通过LOPAT测定试验（莱万的产生、氧化酶、果胶分解和精氨酸二氢水解酶活性、烟草超敏性），橄榄结病的病原体savastanoi长期以来被包括在植物病原性荧光假单胞菌属物种的亚组1b中。萨瓦斯坦假单胞菌致病变种savastanoi与亚组1a中的假单胞菌的不同之处仅在于是果聚糖阴性的。然而，在1990年，在托斯卡纳的橄榄结的传播调查，果聚糖阳性分离的P. savastanoi pv. savastanoi是从佛罗伦萨省（巴尼奥阿里波利）一个果园的两棵橄榄树上的结中分离出来的。几年后，为了评估莱万生产在P. savastanoi pv.在Savastanoi，该调查扩展到随机分布在托斯卡纳的39个其他果园，并且在这些果园中的大约38%中发现果聚糖阳性细菌。表型，基因型和致病性特征允许这些果聚糖阳性分离物被分配到P. savastanoi pv. savastanoi。这些数据表明，在托斯卡纳橄榄果园，果聚糖阳性和果聚糖阴性亚群的这种植物病原细菌可以共存于同一植物。根据所获得的结果，我们建议将LOPAT测定方案的1a和1b亚组结合起来，并将萨伐他诺假单胞菌视为可以是果聚糖阴性或果聚糖阳性的菌种。
Pseudomonas savastanoi pv. savastanoi, the causal agent of olive knot disease, has for a long time been included in subgroup 1b of phytopathogenic, fluorescent Pseudomonas species by the LOPAT determinative tests (production of levan, oxidase, pectinolytic and arginine dihydrolase activity, tobacco hypersensitivity). Pseudomonas savastanoi pv. savastanoi differs from the Pseudomonads in subgroup 1a only in being levan-negative. However, in 1990, during a survey on the spread of olive knot in Tuscany, levan-positive isolates of P. savastanoi pv. savastanoi were isolated from knots on two olive trees in an orchard in the province of Florence (Bagno a Ripoli). Some years later, to assess the further spread of levan production in populations of P. savastanoi pv. savastanoi, the survey was extended to 39 other orchards randomly scattered across Tuscany, and levan-positive bacteria were found in approximately 38% of these orchards. Phenotypic, genotypic and pathogenic characterisation allowed these levan-positive isolates to be assigned to P. savastanoi pv. savastanoi. The data suggest that in Tuscan olive orchards, levan-positive and levan-negative subpopulations of this phytopathogenic bacterium can coexist on the same plant. On the basis of the results obtained we suggest that subgroups 1a and 1b of the LOPAT determinative scheme should be combined, and that P. savastanoi should be considered a bacterial species that can be either levan-negative or levan-positive.