Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21T
Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21T
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DOI：
10.1016/j.btre.2019.e00332
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发表时间：
2019-04
影响因子：
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通讯作者：
Dao Viet Linh;Namiko Gibu;Michiro Tabata;S. Imai;A. Hosoyama;A. Yamazoe;D. Kasai;M. Fukuda
中科院分区：
文献类型：
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作者：
Dao Viet Linh;Namiko Gibu;Michiro Tabata;S. Imai;A. Hosoyama;A. Yamazoe;D. Kasai;M. Fukuda
革兰氏阴性天然橡胶降解剂Rhizobacter gigmiphilusNS21T是从日本植物园土壤中分离得到的，是根杆菌属中新提出的一个种。据报道，latA1基因参与该菌株的天然橡胶降解。为了获得对天然橡胶降解途径的新见解，确定了该菌株的完整基因组序列。 NS21T菌株基因组由6,398,096 bp的环状染色体组成（GenBank登录号CP015118.1），G + C含量为69.72%。基因组包含 5687 个蛋白质编码基因和 68 个 RNA 基因。在预测的基因中，有 4810 个基因被归类为功能性 COG。同源性搜索发现该基因组中存在latA1同源基因(latA2)。定量逆转录PCR和缺失分析表明该菌株的天然橡胶降解需要latA2和latA1。
Gram-negative natural rubber-degrader,Rhizobacter gummiphilusNS21T, which was isolated from soil in the botanical garden in Japan, is a newly proposed species of genus ofRhizobacter. It has been reported that thelatA1gene is involved in the natural rubber degradation in this strain. To gain novel insights into natural rubber degradation pathway, the complete genome sequence of this strain was determined. The genome of strain NS21Tconsists of 6,398,096 bp of circular chromosome (GenBank accession number CP015118.1) with G + C content of 69.72%. The genome contains 5687 protein-coding and 68 RNA genes. Among the predicted genes, 4810 genes were categorized as functional COGs. Homology search revealed that existence oflatA1homologous gene (latA2) in this genome. Quantitative reverse-transcription-PCR and deletion analyses indicated that natural rubber degradation of this strain requireslatA2as well aslatA1.