DNA adduct levels and DNA repair polymorphisms in traffic-exposed workers and a general population sample
DNA adduct levels and DNA repair polymorphisms in traffic-exposed workers and a general population sample
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DOI：
10.1002/ijc.1433
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发表时间：
2001-10-01
影响因子：
6.4
通讯作者：
Peluso, M
中科院分区：
文献类型：
--
作者：
Palli, D;Russo, A;Peluso, M
外周血DNA加合物被认为是靶组织的可接受替代物，并可能预测癌症风险。从佛罗伦萨的EPIC队列中抽取了一组114名暴露于交通污染的工人和100名居民的随机样本，该人群最近显示出DNA加合物水平增加（Palli等人，Int J Cancer 2000;87：444-51）。分别采用P-32后标记法和PCR法对入选时捐献的外周血白细胞进行DNA大加合物和3种DNA修复基因多态性分析。从不吸烟者（p = 0.03）和轻度吸烟者（p = 0.003）中交通工人的加合物水平显著较高。在这两个群体中，城市居民往往比生活在郊区的居民显示出更高的水平，并出现了季节性趋势，加合物水平在夏季最高，冬季最低。与具有2个常见等位基因的工人相比，具有至少1个XPD-Lys 751 Gin多态性变体等位基因的交通工人的水平显着更高（p = 0.02）。多变量分析（调整年龄，季节，居住地区，吸烟，XPD-Lys 751 Gin基因型和抗氧化剂摄入量后）显示职业暴露与较高水平的加合物之间存在显着的2倍相关性（比值比2.1; 95%置信区间1.1-4.2），与最近对类似职位肺癌风险增加的汇总估计一致。我们的研究结果表明，交通工人和一般人口在佛罗伦萨暴露于高水平的遗传毒性剂相关的车辆排放。温暖月份的光化学污染可能是地中海城市化地区遗传毒性损害季节性趋势的原因。(C)2001 Wiley-Liss，Inc.
Peripheral blood DNA adducts have been considered an acceptable surrogate for target tissues and possibly predictive of cancer risk. A group of 114 workers exposed to traffic pollution and a random sample of 100 residents were drawn from the EPIC cohort in Florence, a population recently shown to present increased DNA adduct levels (Palli et of., Int J Cancer 2000;87:444-51). DNA bulky adducts and 3 DNA repair gene polymorphisms were analyzed in peripheral leukocytes donated at enrollment, by using P-32-postlabeling and PCR methods, respectively. Adduct levels were significantly higher for traffic workers among never smokers (p = 0.03) and light current smokers (p = 0.003). In both groups, urban residents tended to show higher levels than those living in suburban areas, and a seasonal trend emerged with adduct levels being highest in summer and lowest in winter. Traffic workers with at least I variant allele for XPD-Lys751 Gin polymorphism had significantly higher levels in comparison to workers with 2 common alleles (p = 0.02). A multivariate analysis (after adjustment for age, season, area of residence, smoking, XPD-Lys751 Gin genotype and antioxidant intake) showed a significant 2-fold association between occupational exposure and higher levels of adducts (odds ratio 2.1; 95% confidence interval 1.1-4.2), in agreement with recent pooled estimates of increased lung cancer risk for similar job titles. Our results suggest that traffic workers and the general population in Florence are exposed to high levels of genotoxic agents related to vehicle emissions. Photochemical pollution in warmer months might be responsible for the seasonal trend of genotoxic damage in this Mediterranean urbanized area. (C) 2001 Wiley-Liss, Inc.