Cirsilineol inhibits proliferation of cancer cells by inducing apoptosis via mitochondrial pathway
Cirsilineol inhibits proliferation of cancer cells by inducing apoptosis via mitochondrial pathway
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Cirsilineol 通过线粒体途径诱导细胞凋亡来抑制癌细胞增殖。
DOI：
10.1211/jpp/60.11.0014
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发表时间：
2008-11-01
影响因子：
3.3
通讯作者：
Xu, Qiang
中科院分区：
文献类型：
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作者：
Sheng, Xia;Sun, Yang;Xu, Qiang
Cirsilineol (4',5-二羟基-3',6,7-三甲氧基黄酮) 是从菊科植物蒿中分离出来的化合物。在这项研究中，我们旨在研究 cirsilineol 对多种类型癌细胞的抗增殖活性及其潜在机制。 Cirsilineol 以浓度依赖性方式显着抑制 Caov-3、Skov-3、PC3 和 Hela 细胞的增殖。通过膜联蛋白 V/碘化丙啶染色测定，该化合物还剂量依赖性地诱导 Caov-3 细胞凋亡。此外，Cirsilineol 诱导线粒体膜电位发生显着变化，并导致细胞色素 c 释放到细胞质中。此外，该化合物引起 caspase-3、caspase-9 和聚（ADP-核糖）聚合酶（PARP）的显着激活。这些结果表明，通过线粒体途径诱导细胞凋亡参与了西西林醇对癌细胞的抗增殖活性。
Cirsilineol (4',5-dihydroxy-3',6,7-trimethoxyflavone) is a compound isolated from the herb of Artemisia vestita Wall (Compositae). In this study, we aimed at examining the anti-proliferative activity of cirsilineol against multiple types of cancer cells and the underlying mechanisms. Cirsilineol significantly inhibited proliferation of Caov-3, Skov-3, PC3 and Hela cells in a concentration-dependent manner. The compound also dose-dependently induced apoptosis in Caov-3 cells, as determined by annexin V/propidium iodide staining. Besides, cirsilineol induced a remarkable change in mitochondrial membrane potential and caused release of cytochrome c to cytosol. Furthermore, the compound caused a marked activation of capase-3, caspase-9 and poly (ADP-ribose) polymerase (PARP). These results suggested that the induction of apoptosis via the mitochondrial pathway was involved in the anti-proliferative activity of cirsilineol against cancer cells.